Fragment detection of coleopteran and triatomine insects in experimentally contaminated acai pulp and sugarcane juice
Rev. Soc. Bras. Med. Trop
;
53: 1-4, dez., 2019. ilus.
Article
in English
| LILACS, SES-SP, SESSP-IDPCPROD, SES-SP
| ID: biblio-1049465
ABSTRACT
INTRODUCTION:
Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects.METHODS:
Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR).RESULTS:
Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food.CONCLUSIONS:
This methodology could be utilized by food analysts to identify possible insect contamination in food samples. (AU)
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Coleoptera
/
Polymerase Chain Reaction
/
Triatominae
/
Euterpe
/
Food
/
Foodborne Diseases
Type of study:
Diagnostic study
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Year:
2019
Type:
Article
Institution/Affiliation country:
Centro de Parasitologia e Micologia/BR
/
Instituto Dante Pazzanese de Cardiologia/BR
/
Laboratório de Microscopia Alimentar/BR
/
Núcleo de Morfologia e Microscopia/BR
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