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CRISPR/Cas9-mediated MSTN gene editing induced mitochondrial alterations in C2C12 myoblast cells
Wang, Lamei; Ma, Sen; Ding, Qiang; Wang, Xiaolong; Chen, Yulin.
  • Wang, Lamei; Northwest A&F University. College of Animal Science and Technology. CN
  • Ma, Sen; Northwest A&F University. College of Animal Science and Technology. CN
  • Ding, Qiang; Northwest A&F University. College of Animal Science and Technology. CN
  • Wang, Xiaolong; Northwest A&F University. College of Animal Science and Technology. CN
  • Chen, Yulin; Northwest A&F University. College of Animal Science and Technology. CN
Electron. j. biotechnol ; 40: 30-39, July. 2019. ilus, graf
Article in English | LILACS | ID: biblio-1053221
ABSTRACT

Background:

Myostatin (MSTN) negatively regulates muscle mass and is a potent regulator of energy metabolism. However, MSTN knockout have affect mitochondrial function. This research assessed the mitochondrial energy metabolism of Mstn−/+ KO cells, and wondered whether the mitochondria biogenesis are affected.

Results:

In this study, we successfully achieved Mstn knockout in skeletal muscle C2C12 cells using a CRISPR/Cas9 system and measured proliferation and differentiation using the Cell-Counting Kit-8 assay and qPCR, respectively. We found that MSTN dysfunction could promote proliferation and differentiation compared with the behaviour of wild-type cells. Moreover, Mstn KO induced an increase in KIF5B expression. The mitochondrial content was significantly increased in Mstn KO C2C12 cells, apparently associated with the increases in PGC-1α, Cox1, Cox2, ND1 and ND2 expression. However, no differences were observed in glucose consumption and lactate production. Interestingly, Mstn KO C2C12 cells showed an increase in IL6 and a decrease in TNF-1α levels.

Conclusion:

These findings indicate that MSTN regulates mitochondrial biogenesis and metabolism. This gene-editing cells provided favourable evidence for animal breeding and metabolic diseases.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Myostatin / Mitochondria Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Northwest A&F University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Myostatin / Mitochondria Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Northwest A&F University/CN