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Antibacterial Activity of Polygonum Orientale Extracts Against Clavibacter Michiganensis subsp. michiganensis, the Agent of Bacterial Canker of Tomato Disease
Cai, Jin; Gao, Yichen; Wang, Mengliang; Zhang, Jing; Zhang, Yongbin; Wang, Qi; Liu, Jiquan.
  • Cai, Jin; Shanxi University. Institute of Applied Chemistry. Taiyuan. CN
  • Gao, Yichen; Shanxi University. School of Life Science. Taiyuan. CN
  • Wang, Mengliang; Shanxi University. Institute of Applied Chemistry. Taiyuan. CN
  • Zhang, Jing; Shanxi University. Institute of Applied Chemistry. Taiyuan. CN
  • Zhang, Yongbin; Shanxi University. Institute of Applied Chemistry. Taiyuan. CN
  • Wang, Qi; Shanxi University. School of Life Science. Taiyuan. CN
  • Liu, Jiquan; Shanxi University of Chinese Medicine. College of Traditional Chinese Medicine. Yuci. CN
Braz. arch. biol. technol ; 62: e19180021, 2019. tab, graf
Article in English | LILACS | ID: biblio-1055397
ABSTRACT
Abstract Clavibacter michiganensis subsp. michiganensis (Cmm), which is a Gram positive bacterium, causes the bacterial canker of tomato disease. The purpose of the study was to evaluate the antibacterial activity of Polygonum orientale extracts against Cmm. In this study, firstly, effects of three extracting parameters (extractive time, extractive temperature, and solid to liquid ratio) of orthogonal experiment design L27 (313) were conducted. Secondly, survival rate was determined and inhibition zone of Cmm rescued post-stress was monitored. Finally, extracellular OD260nm value, extracellular protein content, conformational structure of membrane protein, extracellular alkaline phosphatase (AKP) activity, and ATPase activity were measured to investigate the antibacterial mechanism. Results of orthogonal experiment revealed that extractive time and extractive temperature had highly significant (P<0.01) effects on the antibacterial activity of P. orientale extracts. The optimum conditions were as follows 10h of extractive time, 60°C of extractive temperature, and 120 (gmL) of solid to liquid ratio. This study also demonstrated that the living cells of each sample from survival rate test had almost no resistance or adaptability, and rescued Cmm cells were much easier to be inhibited by P. orientale extracts. The results of antibacterial mechanism indicated that cell membrane and cell wall of Cmm were seriously damaged by P. orientale extracts, and P. orientale extracts reduced the intracellular ATPase activity dramatically. All these findings suggested that P. orientale extracts had a strong antibacterial activity to inhibit Cmm, and could be used for the ecological management of the bacterial canker of tomato disease.
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Full text: Available Index: LILACS (Americas) Main subject: Plant Diseases / Pest Control, Biological / Phytochemicals / Anti-Bacterial Agents Type of study: Evaluation studies Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Shanxi University of Chinese Medicine/CN / Shanxi University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Plant Diseases / Pest Control, Biological / Phytochemicals / Anti-Bacterial Agents Type of study: Evaluation studies Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Shanxi University of Chinese Medicine/CN / Shanxi University/CN