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Bubaline Diaphragm Matrix: Development and Clinical Assessment into Cattle Abdominal Hernia Repair
Department of Veterinary Surgery and RadiologyVora, Shruti Dineshbhai; Department of Veterinary Surgery and RadiologyKumar, Vineet; Department of Veterinary Surgery and RadiologyAsodiya, Foram Arvindbhai; Department of Veterinary Physiology and BiochemistrySingh, Vivek Kumar; Department of Veterinary PathologyFefar, Dhaval Tribhovanbhai; Gajera, Harsukh Popatbhai.
  • Department of Veterinary Surgery and RadiologyVora, Shruti Dineshbhai; Junagadh Agricultural University. College of Veterinary Science and Animal Husbandry. Department of Veterinary Surgery and RadiologyVora, Shruti Dineshbhai. Junagadh. IN
  • Department of Veterinary Surgery and RadiologyKumar, Vineet; Junagadh Agricultural University. College of Veterinary Science and Animal Husbandry. Department of Veterinary Surgery and RadiologyKumar, Vineet. Junagadh. IN
  • Department of Veterinary Surgery and RadiologyAsodiya, Foram Arvindbhai; Junagadh Agricultural University. College of Veterinary Science and Animal Husbandry. Department of Veterinary Surgery and RadiologyAsodiya, Foram Arvindbhai. Junagadh. IN
  • Department of Veterinary Physiology and BiochemistrySingh, Vivek Kumar; Junagadh Agricultural University. College of Veterinary Science and Animal Husbandry. Department of Veterinary Physiology and BiochemistrySingh, Vivek Kumar. Junagadh. IN
  • Department of Veterinary PathologyFefar, Dhaval Tribhovanbhai; Junagadh Agricultural University. College of Veterinary Science and Animal Husbandry. Department of Veterinary PathologyFefar, Dhaval Tribhovanbhai. Junagadh. IN
  • Gajera, Harsukh Popatbhai; Junagadh Agricultural University. Department of Biotechnology. Junagadh. IN
Braz. arch. biol. technol ; 62: e19180442, 2019. tab, graf
Article in English | LILACS | ID: biblio-1055411
ABSTRACT
Abstract The purpose of the study was to develop a xenogenic bubaline diaphragm matrix (BDM) for abdominal hernia repair. A fresh diaphragm was decellularized using aqueous sodium dodecyl sulfate (SDS) solutions (0.5-4% w/v) over a period. Acellularity was confirmed histologically and characterized by Masson's trichrome staining, scanning electron microscopy (SEM), DNA quantification, agarose gel electrophoresis, and Fourier-transform infrared spectroscopy. The BDM was used for clinical abdominal hernia repair in six cattle. Clinical, hemato-biochemical and antioxidant parameters were evaluated to assess biocompatibility of xenogenic BDM. Histologically, the diaphragm treated with 2% SDS for 48 h showed complete acellularity and orderly arranged collagen fibers. The SEM confirmed preservation of collagen structure and integrity. The DNA content was significantly (P < 0.05) reduced in BDM (33.12 ± 5.40 ng/mg) as compared to the native diaphragm (443.96 ± 162.60 ng/mg). DNA extracts from BDM show considerable removal of DNA material, with absence of DNA band in agarose gel. The FTIR spectrum of BDM has shown all characteristic transmittance peaks of bovine skin collagen indicating preserved collagen structure. Six cattle with BDM implant recovered uneventfully and remained sound at least upto 6 months. Hemato-biochemical and antioxidant findings were unremarkable. Bubaline diaphragm matrix shows excellent repair efficiency and biocompatibility for abdominal hernia repair in cattle without complications.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Buffaloes / Diaphragm / Hernia, Abdominal Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2019 Type: Article Affiliation country: India Institution/Affiliation country: Junagadh Agricultural University/IN

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Full text: Available Index: LILACS (Americas) Main subject: Buffaloes / Diaphragm / Hernia, Abdominal Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2019 Type: Article Affiliation country: India Institution/Affiliation country: Junagadh Agricultural University/IN