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Pam2CSK4 (TLR2 agonist) induces periodontal destruction in mice
Department of PeriodontologySOUZA, João Antonio Chaves de; MAGALHÃES, Fernando Augusto Cintra; Department of PeriodontologyOLIVEIRA, Guilherme Jose Pimentel Lopes de; Department of Diagnosis and SurgeryDE MOLON, Rafael Scaf; Department of Physiology and PathologyZUANON, José Antonio; Department of StomatologySOUZA, Pedro Paulo Chaves de.
  • Department of PeriodontologySOUZA, João Antonio Chaves de; Universidade Federal de Goiás. School of Dentistry. Department of PeriodontologySOUZA, João Antonio Chaves de. Goiania. BR
  • MAGALHÃES, Fernando Augusto Cintra; Universidade Federal do Maranhão. Department of Nursing. Imperatriz. BR
  • Department of PeriodontologyOLIVEIRA, Guilherme Jose Pimentel Lopes de; Universidade Federal de Uberlândia. School of Dentistry. Department of PeriodontologyOLIVEIRA, Guilherme Jose Pimentel Lopes de. Uberlândia. BR
  • Department of Diagnosis and SurgeryDE MOLON, Rafael Scaf; Universidade Estadual Paulista. School of Dentistry. Department of Diagnosis and SurgeryDE MOLON, Rafael Scaf. Araraquara. BR
  • Department of Physiology and PathologyZUANON, José Antonio; Universidade Estadual Paulista. School of Dentistry. Department of Physiology and PathologyZUANON, José Antonio. Araraquara. BR
  • Department of StomatologySOUZA, Pedro Paulo Chaves de; Universidade Federal de Goiás. School of Dentistry. Department of StomatologySOUZA, Pedro Paulo Chaves de. Goiania. BR
Braz. oral res. (Online) ; 34: e012, 2020. graf
Article in English | LILACS | ID: biblio-1055530
ABSTRACT
Abstract Lipoproteins are important bacterial immunostimulating molecules capable of inducing receptor activator of nuclear factor-κB (RANKL) and osteoclast formation in vitro and in vivo . Although these molecules are present in periodontopathogenic bacteria, their role in periodontitis is not known. In this study, we used Pam2CSK4 (PAM2), a synthetic molecule that mimics bacterial lipoprotein, to investigate the effects of lipoproteins on periodontitis in mice. C57BL/6 male mice were randomly divided into three experimental groups 1) Negative control group animals received vehicle injection; 2) Positive control group animals received injection of Escherichia coli lipopolysaccharide (LPS); 3) PAM2 group animals received PAM2 injection. All the injections were performed bilaterally every other day into the palatal mucosa between first and second molars. After twenty-four days, the animals were euthanized to assess alveolar bone volume (micro-CT), cellular and extracellular composition in the gingiva (stereometric analysis), and osteoclast numbers (TRAP staining). Treatment with either PAM2 or LPS induced gingival inflammation, as demonstrated by increased infiltration of inflammatory cells and enhanced angiogenesis, associated with a smaller number of fibroblasts and decreased extracellular matrix. Importantly, treatment not only with LPS but also with PAM2 resulted in a larger number of TRAP+ multinucleated osteoclasts and significant loss of alveolar bone. Collectively, our data demonstrate that PAM2 can induce gingival inflammation and bone loss in mice, broadening the avenues of investigation into the role of lipoproteins in the pathogenesis of periodontal disease.
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Full text: Available Index: LILACS (Americas) Main subject: Periodontitis / Toll-Like Receptor 2 / Lipopeptides Type of study: Etiology study / Prognostic study Limits: Animals Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade Federal de Goiás/BR / Universidade Federal de Uberlândia/BR / Universidade Federal do Maranhão/BR

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Full text: Available Index: LILACS (Americas) Main subject: Periodontitis / Toll-Like Receptor 2 / Lipopeptides Type of study: Etiology study / Prognostic study Limits: Animals Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade Federal de Goiás/BR / Universidade Federal de Uberlândia/BR / Universidade Federal do Maranhão/BR