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Protective effect of dietary vitamin E (alpha; Tocopherol) on artemisinin induced oxidative liver tissue damage in rats / Efecto protector de la vitamina E en la dieta (alfa; tocoferol) sobre el daño oxidativo del tejido hepático inducido por artemisinina en ratas
Zaki, Mohamed Samir Ahmed; Haidara, Mohamed A; Alghamd, Mansour A; Shati, Ayed A; Wares, Adnan; Eid, Refaat A.
  • Zaki, Mohamed Samir Ahmed; King Khalid University. College of Medicine. Anatomy Department. Abha. SA
  • Haidara, Mohamed A; Cairo University. Kasr al-Aini Faculty of Medicine. Physiology Department. Cairo. EG
  • Alghamd, Mansour A; King Khalid University. College of Medicine. Anatomy Department. Abha. SA
  • Shati, Ayed A; King Khalid University. College of Medicine. Department of Child Health. Abha. SA
  • Wares, Adnan; King Khalid University. College of Medicine. Anatomy Department. Abha. SA
  • Eid, Refaat A; King Khalid University. College of Medicine. Pathology Department. Abha. SA
Int. j. morphol ; 38(2): 278-288, abr. 2020. graf
Article in English | LILACS | ID: biblio-1056435
Responsible library: CL1.1
ABSTRACT
This experiment was designed to study the effects of oral administration of artemether which is the most rapid-acting class of antimalarial drugs and the possible protective effect of vitamin E taken with it on the liver of albino rats. A total of twenty-four adult male albino rats were used in this study and were divided into four groups. Group one served as a control and rats in group two exposed to oral intake of artemether daily for fifteen days. The third and fourth groups treated with artemether plus low and high doses of vitamin E respectively. At the end of the experiment, the rats were sacrificed, and the livers were obtained and processed for histological, biochemical and statistical studies. Histological study of the hepatocytes of rats exposed to artemether showed nearly complete disintegration of most cellular contents except few numbers of mitochondria and rough endoplasmic reticulum. Also, the cytoplasm of these cells had few lysosomes, many vacuoles and irregular nuclei with abnormal distribution of chromatin and were shown. The hepatic sinusoids were dilated and filled with blood and vacuoles and bile ductules were abnormal in its structure. Treatment with low and high doses of vitamin E in concomitant with artemether ameliorated the hepatic histopathological lesions and its parenchyma attained nearly normal structure. As far as biochemical changes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in rats treated with artemether were significantly elevated as compared to the control. Superoxide dismutase (SOD) and malondialdehyde (MDA) levels were significantly increased in the liver in rats treated with artemether. However, vitamin E ameliorated the rise in ALT and AST with decreased MDA concentration and levels of SOD as compared to the corresponding artemether group values. Results of the present suggest that artemether has a harmful and stressful effect on hepatic tissue and the treatment with vitamin E may alleviate this toxicity.
RESUMEN
Este experimento fue diseñado para estudiar los efectos de la administración oral de arteméter, la clase de medicamentos antipalúdicos de acción rápida, y el posible efecto protector de la vitamina E en el hígado de ratas albinas. Se utilizaron un total de 24 ratas albinas machos adultas y se dividieron en cuatro grupos. El grupo uno sirvió como control y las ratas en el grupo dos recibieron la dosis oral de arteméter diariamente durante 15 días. Los grupos tres y cuatro fueron tratados con arteméter, más dosis bajas y altas de vitamina E, respectivamente. Al final del experimento, se sacrificaron las ratas y se obtuvieron y procesaron los hígados para estudios histológicos, bioquímicos y estadísticos. El estudio histológico de los hepatocitos de ratas expuestas a arteméter mostró una desintegración casi completa de la mayoría de los contenidos celulares, excepto algunos mitocondrias y retículo endoplásmico rugoso. Además, el citoplasma de estas células tenía pocos lisosomas, muchas vacuolas y núcleos irregulares con distribución anormal de cromatina. Los sinusoides hepáticos estaban dilatados y llenos de sangre y vacuolas, y los conductos biliares tenían una estructura anormal. El tratamiento con dosis bajas y altas de vitamina E en forma concomitante con arteméter mejoró las lesiones histopatológicas hepáticas y su parénquima alcanzó una estructura casi normal. En cuanto a los cambios bioquímicos, la alanina aminotransferasa (ALT) y la aspartato aminotransferasa (AST) en ratas tratadas con arteméter se elevaron significativamente en comparación con el control. Los niveles de superóxido dismutasa (SOD) y malondialdehído (MDA) aumentaron significativamente en el hígado en ratas tratadas con arteméter. Sin embargo, la vitamina E mejoró el aumento de ALT y AST con una disminución de la concentración de MDA y los niveles de SOD en comparación con los valores correspondientes del grupo de arteméter. Los resultados del presente estudio sugieren que el arteméter tiene un efecto dañino y estresante sobre el tejido hepático y el tratamiento con vitamina E puede aliviar esta toxicidad.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Vitamin E / Artemisinins / Chemical and Drug Induced Liver Injury, Chronic Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: Egypt / Saudi Arabia Institution/Affiliation country: Cairo University/EG / King Khalid University/SA

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Full text: Available Index: LILACS (Americas) Main subject: Vitamin E / Artemisinins / Chemical and Drug Induced Liver Injury, Chronic Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: Egypt / Saudi Arabia Institution/Affiliation country: Cairo University/EG / King Khalid University/SA