Antibacterial Effects of Cuminum cyminum Extract Against Enterococcus Faecalis Biofilms From Clinical Isolates
Pesqui. bras. odontopediatria clín. integr
;
19: e5115, 2019. tab
Article
in English
| LILACS, BBO
| ID: biblio-1056831
ABSTRACT
Abstract Objective:
To compare the antibacterial efficacy of Cuminum cyminum (cumin) extract and 2% chlorhexidine. Material andMethods:
E. faecalis was isolated from non-vital teeth with chronic apical abscess. Samples were then bred in the ChromAgar medium. Subsequently, E. faecalis bacteria's DNA extraction was performed. DNA was then amplified by conventional PCR, and the product was run on an electrophoresis gel. Subsequently, we extracted Cuminumcyminum seeds using the steam distillation technique. The extract was diluted at various concentrations 0.2, 0.5, 0.7, 1.0, and 1.2 mg/mL.The extract's antibacterial effect was evaluated using an ELISA reader with optical density. Specifically, we assessed the turbidity of E. faecalis in biofilms following immersion in antibacterial agentsResults:
In the clinically isolated E. faecalis group, the OD values of 0.7 and 1.0 mg/mL cumin extracts were significantly different from that of 0.2 mg/mL cumin extract. A significant difference was also observed between the OD values of 1.0 mg/mL cumin extract and 2% CHX (p<0.05)Conclusion:
The antibacterial effect of 1.0 mg/mL Cuminum cyminum extract had higher efficiency than 2% chlorhexidine against E. faecalis biofilms from clinical isolates.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Plants, Medicinal
/
Chlorhexidine
/
Enterococcus faecalis
/
Biofilms
/
Cuminum
Country/Region as subject:
Asia
Language:
English
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
Dentistry
Year:
2019
Type:
Article
Affiliation country:
Indonesia
Institution/Affiliation country:
Universitas Indonesia/ID
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