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Phage therapy as an approach to control salmonella enterica serotype enteritidis infection in mice
Dallal, Mohammad Mehdi Soltan; Nikkhahi, Farhad; Alimohammadi, Mahmoud; Douraghi, Masoumeh; Rajabi, Zahra; Foroushani, Abbas Rahimi; Azimi, Akram; Fardsanei, Fatemeh.
  • Dallal, Mohammad Mehdi Soltan; School of Public Health (TUMS). Department of Pathobiology. Division of Food Microbiology. Tehran. IR
  • Nikkhahi, Farhad; School of Public Health (TUMS). Department of Pathobiology. Division of Medical Bacteriology. Tehran. IR
  • Alimohammadi, Mahmoud; Tehran University of Medical Sciences. School of Public Health. Environmental Health Engineering Department. Tehran. IR
  • Douraghi, Masoumeh; Tehran University of Medical Sciences (TUMS). Food Microbiology Research Center. Tehran. IR
  • Rajabi, Zahra; Tehran University of Medical Sciences (TUMS). Food Microbiology Research Center. Tehran. IR
  • Foroushani, Abbas Rahimi; Tehran University of Medical Sciences. School of Public Health. Department of Epidemiology and Biostatistics. Tehran. IR
  • Azimi, Akram; Tehran University of Medical Sciences (TUMS). Food Microbiology Research Center. Tehran. IR
  • Fardsanei, Fatemeh; School of Public Health (TUMS). Department of Pathobiology. Division of Medical Bacteriology. Tehran. IR
Rev. Soc. Bras. Med. Trop ; 52: e20190290, 2019. graf
Article in English | LILACS | ID: biblio-1057257
ABSTRACT
Abstract

INTRODUCTION:

Salmonella enterica serotype Enteritidis (S. Enteritidis) is a cause of food-borne human illness. Given the prevalence of antibiotic resistance of Salmonella Enteritidis and the lack of antibiotic efficacy in future years, its replacement with other agents is necessary. One of the most useful agents is bacteriophages. METHODS S. Enteritidis was identified using a multiplex polymerase chain reaction assay. The effective bacteriophages were isolated from hospital wastewater samples. The effects of the bacteriophages were evaluated both in vitro and in vivo. RESULTS The phage SE20 belonged to the Podoviridae family, and the genome size was 40 kb. The evaluation of phage SE20 at variable pH ranges showed its susceptibility to pH < 3 and pH > 12. The animal model showed that mice infected with S. Enteritidis developed hepatomegaly and splenomegaly, but did not experience gastrointestinal complications after receiving the bacteriophages. CONCLUSIONS The results of this study suggest that phage SE20 is a promising candidate for controlling salmonellosis caused by Salmonella Enteritidis.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Salmonella enteritidis / Salmonella Infections / Phage Therapy Type of study: Prognostic study / Risk factors Limits: Animals Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2019 Type: Article Affiliation country: Iran Institution/Affiliation country: School of Public Health (TUMS)/IR / Tehran University of Medical Sciences (TUMS)/IR / Tehran University of Medical Sciences/IR

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Full text: Available Index: LILACS (Americas) Main subject: Salmonella enteritidis / Salmonella Infections / Phage Therapy Type of study: Prognostic study / Risk factors Limits: Animals Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2019 Type: Article Affiliation country: Iran Institution/Affiliation country: School of Public Health (TUMS)/IR / Tehran University of Medical Sciences (TUMS)/IR / Tehran University of Medical Sciences/IR