Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis
Braz. j. biol
;
34(1): 5-7, 2003.
Article
in English
| LILACS, SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1061606
ABSTRACT
Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinalattachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry andimmunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizesthe different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection.Besides, immuno-dot assay seems to be a possible alternative as a capture method.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Antibodies
Type of study:
Diagnostic study
Language:
English
Journal:
Braz. j. biol
Year:
2003
Type:
Article
Institution/Affiliation country:
Instituto Butantan/BR
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