Your browser doesn't support javascript.
loading
Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri
Laia, Marcelo Luiz de; Moreira, Leandro Marcio; Fernandes Gonçalves, Janaína; Tiraboschi Ferro, Maria Inês; Pinto Rodrigues, Any Caroliny; Santos, Jéssica Naiara dos; Barbosa Felestrino, Érica; Aparecido Ferro, Jesus.
  • Laia, Marcelo Luiz de; Universidade Federal dos Vales do Jequitinhonha e Mucuri. Faculdade de Ciências Agrárias. Departamento de Engenharia Florestal. Diamantina. BR
  • Moreira, Leandro Marcio; Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Ciências Biológicas. Ouro Preto. BR
  • Fernandes Gonçalves, Janaína; Universidade Federal dos Vales do Jequitinhonha e Mucuri. Instituto de Ciências Agrárias. Unaí. BR
  • Tiraboschi Ferro, Maria Inês; Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Tecnologia. Jaboticabal. BR
  • Pinto Rodrigues, Any Caroliny; Universidade Federal dos Vales do Jequitinhonha e Mucuri. Faculdade de Ciências Agrárias. Departamento de Engenharia Florestal. Diamantina. BR
  • Santos, Jéssica Naiara dos; Universidade Federal dos Vales do Jequitinhonha e Mucuri. Faculdade de Ciências Agrárias. Departamento de Engenharia Florestal. Diamantina. BR
  • Barbosa Felestrino, Érica; Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto. BR
  • Aparecido Ferro, Jesus; Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Tecnologia. Jaboticabal. BR
Electron. j. biotechnol ; 42: 30-41, Nov. 2019. tab, graf, ilus
Article in English | LILACS | ID: biblio-1087456
ABSTRACT

Background:

Gene expression analysis via microarray is widely used in phytobacteria to validate differential gene expression associated with virulence or to compare biological profiles of wild type and mutant strains. Here, we employed DNA microarrays to study the early stages of the infection process (24, 72 and 120 h post-inoculation) of Xanthomonas citri subsp. citri (Xac) infecting Citrus sinensis to interrogate the expression profiles of hypothetical genes.

Results:

Under infective conditions, 446 genes were up- and 306 downregulated. Outstanding among genes upregulated during infection were those involved in synthesizing the Type 3 Secretion System and effectors, xanthan gum and quorum-sensing induction, and flagellum synthesis and regulation. Additionally, 161 hypothetical genes were up- and 100 were downregulated, 49 of which are known to have a significant biological role. To understand hypothetical gene co-regulation or -expression, nine expression profiles including 158 genes were identified during the three infection phases. Of these, 47 hypothetical genes were identified as having expression profiles associated with at least one connected to a gene associated with adaptation and virulence.

Conclusions:

Expression patterns of six differentially expressed genes were validated by quantitative reverse transcription polymerase chain reaction, thus demonstrating the effectiveness of this tool in global gene expression analysis in Xac.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Xanthomonas / Citrus sinensis Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade Federal de Ouro Preto/BR / Universidade Federal dos Vales do Jequitinhonha e Mucuri/BR

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Xanthomonas / Citrus sinensis Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade Federal de Ouro Preto/BR / Universidade Federal dos Vales do Jequitinhonha e Mucuri/BR