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Association between genetic polymorphisms in rank, rankl and opg and peri-implant diseases in patients from the Amazon Region
Silva, Raphael Carvalho e; Reis, Marilia Bianchini Lemos; Arid, Juliana; Flores, Elvira Katherine Barriga; Cruz, Giuseppe Valduga; Marañón-Vásquez, Guido A; Souza, Lizete Karla Filgueiras de; Novaes Jr, Arthur Belém; Queiroz, Alexandra Mussolino de; Küchler, Erika Calvano.
  • Silva, Raphael Carvalho e; Faculdade do Amazonas. Department of Oral and Maxillofacial Surgery and Dental Implants. Manaus. BR
  • Reis, Marilia Bianchini Lemos; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Oral and Maxillofacial Surgery and Periodontology. Ribeirão Preto. BR
  • Arid, Juliana; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry. Ribeirão Preto. BR
  • Flores, Elvira Katherine Barriga; Universidade Positivo. School of Health Science. Curitiba. BR
  • Cruz, Giuseppe Valduga; Universidade Positivo. School of Health Science. Curitiba. BR
  • Marañón-Vásquez, Guido A; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry. Ribeirão Preto. BR
  • Souza, Lizete Karla Filgueiras de; Faculdade do Amazonas. Department of Oral and Maxillofacial Surgery and Dental Implants. Manaus. BR
  • Novaes Jr, Arthur Belém; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Oral and Maxillofacial Surgery and Periodontology. Ribeirão Preto. BR
  • Queiroz, Alexandra Mussolino de; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry. Ribeirão Preto. BR
  • Küchler, Erika Calvano; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry. Ribeirão Preto. BR
Braz. dent. j ; 31(1): 63-68, Jan.-Feb. 2020. tab, graf
Article in English | LILACS | ID: biblio-1089266
ABSTRACT
Abstract The present study evaluated polymorphisms in RANK, RANKL and OPG-encoding genes to assess whether they are associated with mucositis and peri-implantitis in a population from the Brazilian Amazon region. One hundred and fourteen patients with dental implants were included in the study. After clinical and radiographic examination, the sample was categorized into 4 groups, according to the peri-implant status Healthy (n=71), Mucositis (n=30), Peri-implantitis (n=13) and Diseased (Mucositis + Peri-implantitis, n=43). Genomic DNA was extracted from buccal cells from saliva, and the genetic polymorphism in osteoprotegerin (OPG), Kappa nuclear factor activator receptor (RANKL) and nuclear kappa factor activator receptor (RANK) were genotyped by the real time PCR. Univariate and multivariate statistical analyses were performed to compare clinical variables among groups and to evaluate genotypes and alleles distributions and the established alpha was 5%. Age, peri-implant biotype, diabetes and presence of peri-implant biofilm were associated with mucositis (p<0.05) and peri-implantitis (p<0.05). Smoking, alcoholism, and periodontal biofilms were also associated with the presence of peri-implantitis (p<0.05). Univariate and multivariate analysis did not demonstrate an association of peri-implantitis or mucositis with any genetic polymorphism in RANK (rs3826620), RANKL (rs9594738) and OPG (rs2073618) (p>0.05). The studied genetic polymorphism in RANK, RANKL and OPG were not associated with mucositis and peri-implantitis in a Brazilian population from the Amazon region.
RESUMO
Resumo O presente estudo avaliou a associação da predisposição clínica e dos fatores genéticos com a presença de doenças peri-implantares. Cento e quatorze pacientes com implantes dentais instalados na Clínica de Especialização do Amazonas, Brazil, foram incluidos no estudo. Após exame clínico e radiográfico, a amostra foi categorizada em 4 grupos, de acordo com o Status peri-implantar saúde (n=71), mucosite (n=30), peri-implantite (n=13) e doentes (mucosite + peri-implantite). DNA genômico foi extraído de células orais da saliva, e o polimorfismo genético em osteoprotegerina (OPG), ligante do receptor ativador do fator Kappa nuclear (RANKL) e receptor ativador do fator Kappa nuclear (RANK) foram genotipados por PCR em tempo real. O estudo se propôs a avaliar se os polimorfismos em RANK, RANKL e OPG estão envolvidos na patogênese da mucosite e da peri-implantite, e avaliar também a presença de fatores de risco moduladores da resposta em uma população brasileira. Idade, biotipo peri-implantar, diabetes e presença de biofilme peri-implantar foram associados a mucosite (p<0.05) e peri-implantite (p<0.05). Tabagismo, alcoolismo e biofilme periodontal também foram associados com a presença de peri-implantite (p<0.05). Análise univariada e multivariada não demonstraram associação de peri-implantite ou mucosite com os polimorfismos genéticos em RANK (rs3826620), RANKL (rs9594738) e OPG (rs2073618) (p>0.05). Os polimorfismos genéticos estudados não foram associados com mucosite e peri-implantite em uma população brasileira da região Amazônica.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Dental Implants / RANK Ligand / Receptor Activator of Nuclear Factor-kappa B / Osteoprotegerin / Peri-Implantitis Type of study: Risk factors Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdade do Amazonas/BR / Universidade Positivo/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Dental Implants / RANK Ligand / Receptor Activator of Nuclear Factor-kappa B / Osteoprotegerin / Peri-Implantitis Type of study: Risk factors Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdade do Amazonas/BR / Universidade Positivo/BR / Universidade de São Paulo/BR