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Effects of NO/cGMP inhibitors in a rat model of anaphylactoid shock
Albuquerque, A A S; Ferreira, L G; Carvalho, M T M; Capellini, V K; Evora, P R B; Celotto, A C.
  • Albuquerque, A A S; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Cirurgia e Anatomia. Ribeirão Preto. BR
  • Ferreira, L G; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Cirurgia e Anatomia. Ribeirão Preto. BR
  • Carvalho, M T M; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Cirurgia e Anatomia. Ribeirão Preto. BR
  • Capellini, V K; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Cirurgia e Anatomia. Ribeirão Preto. BR
  • Evora, P R B; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Cirurgia e Anatomia. Ribeirão Preto. BR
  • Celotto, A C; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Cirurgia e Anatomia. Ribeirão Preto. BR
Braz. j. med. biol. res ; 53(3): e8853, 2020. tab, graf
Article in English | LILACS | ID: biblio-1089343
Responsible library: BR1.1
ABSTRACT
Anaphylactic shock can be defined as an acute syndrome, and it is the most severe clinical manifestation of allergic diseases. Anaphylactoid reactions are similar to anaphylactic events but differ in the pathophysiological mechanism. Nitric oxide (NO) inhibitors during anaphylaxis suggest that NO might decrease the signs and symptoms of anaphylaxis but exacerbate associated vasodilation. Therefore, blocking the effects of NO on vascular smooth muscle by inhibiting the guanylate cyclase (GC) would be a reasonable strategy. This study aimed to investigate the effects of NO/cGMP pathway inhibitors methylene blue (MB), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME), and indigo carmine (IC) in shock induced by compound 48/80 (C48/80) in rats. The effect was assessed by invasive blood pressure measurement. Shock was initiated by C48/80 intravenous bolus injection 5 min before (prophylactic) or after (treatment) the administration of the inhibitors MB (3 mg/kg), L-NAME (1 mg/kg), and IC (3 mg/kg). Of the groups that received drugs as prophylaxis for shock, only the IC group did not present the final systolic blood pressure (SBP) better than the C48/80 group. Regarding shock treatment with the drugs tested, all groups had the final SBP similar to the C48/80group. Altogether, our results suggested that inhibition of GC and NO synthase in NO production pathway was not sufficient to revert hypotension or significantly improve survival.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Cyclic GMP / Enzyme Inhibitors / Anaphylaxis / Muscle, Smooth, Vascular / Nitric Oxide Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

Full text: Available Index: LILACS (Americas) Main subject: Cyclic GMP / Enzyme Inhibitors / Anaphylaxis / Muscle, Smooth, Vascular / Nitric Oxide Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR