Aislamiento de Salmonella spp. a partir de huevos de gallina para consumo comercializados en la ciudad de Casilda, Santa Fe, Argentina

RESUMEN

Con el objetivo de determinar la presencia de Salmonella spp. en huevos destinados al consumo humano en la ciudad de Casilda, Santa Fe, Argentina, se recolectaron 125 huevos de gallina obtenidos en comercios de la ciudad y se muestrearon yemas y cáscaras. Ambas muestras se sembraron en caldo tetrationato con el agregado de solución de yodo y verde brillante y se incubaron a 43º C durante 24 horas. De cada medio sembrado se tomó una alícuota de la superficie y se sembró en agar Xilosa-Lisina-Desoxicolato, incubándose durante 24-48 horas a 37 °C. Las colonias desarrolladas se identificaron utilizando pruebas bioquímicas y aquellas identificadas como Salmonella spp. se serotipificaron. Se aislaron 3 cepas de Salmonella spp: Salmonella ser. Tiphymurium y Salmonella ser. Anatum a partir de muestras de yema y Salmonella ser. Anatum a partir de la cáscara de un tercer huevo. El aislamiento de Salmonella spp. en huevos para consumo humano refuerza la necesidad de tomar medidas de bioseguridad para reducir la incidencia de esta bacteria en las granjas.

ABSTRACT

In order to determine the presence of Salmonella spp. in eggs from Casilda city, Santa Fe, Argentina, a total of 125 eggs were collected from different stores and yolks and egg shells were sampled. Both samples were seeded in tetrathionate broth with the addition of iodine and brilliant green solution and incubated at 43 ° C for 24 hours. An aliquot of the surface was taken from each medium seeded and seeded on Xylose-Lysine-Deoxycholate agar, being incubated for 24-48 hours at 37 ° C. The developed colonies were identified using biochemical tests and those identified as Salmonella spp. they were serotyped. Three strains of Salmonella spp. were isolated: Salmonella ser. Tiphymurium and Salmonella ser. Anatum from samples of yolk and Salmonella ser. Anatum from the egg shell of a third egg. The isolation of Salmonella spp. in eggs for human consumption reinforces the need to take biosecurity measures to reduce the incidence of this bacterium in the farms.