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Molecular characterization of carbapenem-resistant Acinetobacter baumannii strains from a tertiary care center in South India / Caracterización molecular de cepas de Acinetobacter baumannii carbapenem-resistante en un centro de atención terciaria en el sur de la India
Kalal, Bhuvanesh Sukhlal; Chandran, Salesh P; Yoganand, Raksha; Nagaraj, Savitha.
  • Kalal, Bhuvanesh Sukhlal; A. J. Institute of Medical Sciences and Research Centre. A. J. Research Centre. Mangaluru. IN
  • Chandran, Salesh P; HLL Biotech Ltd. Chennai. IN
  • Yoganand, Raksha; Employees State Insurance Corporation Medical College and Post Graduate Institute of Medical Science and Research. Bengaluru. IN
  • Nagaraj, Savitha; St. John's Medical College Hospital. Department of Microbiology. Bengaluru. IN
Infectio ; 24(1): 27-34, ene.-mar. 2020. tab, graf
Article in English | LILACS, COLNAL | ID: biblio-1090540
ABSTRACT

Objectives:

Carbapenem resistantAcinetobacter baumannii is an important therapeutic and infection control challenge worldwide. In this study, we investigated the prevalence and distribution of molecular mechanisms of resistance among carbapenem resistant A. baumannii species at a tertiary care setting in South India. Materials and

Methods:

A total of 89 non-duplicate clinical isolates of carbapenem-resistantA. baumannii were collected from critical care units of St. John's Medical College Hospital, Bengaluru, India. Polymerase chain reaction (PCR) was performed to detect blaOXA type carbapenemase blaOXA-51-like, blaOXA-23-like, blaOXA-24-like and bla OXA-58-like, MBL genes blaNDM, blaIMP, and blaVIM genes. Molecular typing of carbapenem-resistant A. baumannii strains was performed by using Rep-PCR.

Results:

Eighty-seven of the isolates were found to carry the blaOXA-51 gene and 81 (91%) isolates were found to have blaOXA-51-like gene and blaOXA-23, gene. The bla OXA-24 like gene was detected in two isolates of which one also carried blaOXA-51 like and one isolate carried blaVIM coding gene. The prevalence of blaNDM, blaIMP, bla VIM genes was 12(13%),14 (16%) and 57(64%) respectively. Cluster analyses revealed a 90% similarity and were divided into 5 clusters. Most of the isolates containing carbapenemases coding genes grouped under cluster A, C and UC. Considerable heterogeneity was observed within UC cluster indicating circulation of multiple strains of A. baumannii within our institution.

Conclusions:

Carbapenemase coding blaOXA-23, blaOXA-24 and blaOXA-51 -like were more common than blaVIM and blaNDM. The presence of blaNDM with other genes coding for carbapenemases indicate the ability of the strains to acquire novel genes despite having its share of the blaOXA like carbapenemase.
RESUMEN

Objetivos:

El Acinetobacter baumannii resistente a Carbapenem es un reto importante en todo el mundo para su tratamiento y para el control de infecciones hospitalarias. Nosotros estudiamos la prevalencia y los mecanismos de resistencia en aislados de un centro de atención terciario, en el sur de la India Materiales y

Métodos:

Se estudiaron 89 aislados clínicos de A. baumannii recolectados en unidades de cuidado crítico del Hospital St. John's Medical College en Bengaluru, India. Se realizó amplificación por PCR (Reacción en Cadena de Polimerasa) y luego tipificación molecular con la técnica Rep-PCR (PCR de elementos repetitivos palindromicos) para detectar los genes de carbapenemasa blaOXA, blaOXA-51, blaOXA-23, blaOXA-24, blaOXA-58, MBL, blaNDM, blaIMP y blaVIM.

Resultados:

Se encontraron 87 aislados que llevaban el gen blaOXA-51 y de ellos en 81 (91%) se encontró blaOXA-51 y blaOXA-23. El blaOXA-24 se detectó en dos aislados de los cuales uno de ellos llevaba blaOXA-51 y otro blaVIM. Los genes blaNDM, blaIMP y blaVIM se encontraron en 12 (13%),14 (16%) y 57(64%) de los aislados, respectivamente. El análisis de agrupamiento reveló un 90% de similitud entre los aislados y que podían asignarse a 5 agrupamientos. La mayoría de aislados llevaban genes de carbapenemasas de los grupos A, C y UC. Se observó mucha heterogeneidad dentro del agrupamiento UC indicando que existe circulación de múltiples cepas de A. baumannii dentro de nuestra institución.

Conclusiones:

Las carbapenemasas que codifican para blaOXA-23, blaOXA-24 y blaOXA-51 son más comunes que blaVIM y blaNDM en nuestra institución. La presencia de NDM con otros genes codificando para carbapenemasas indica la capacidad que tienen este tipo de aislados para adquirir nuevos genes a pesar de contar con blaOXA.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Carbapenems / Acinetobacter baumannii Type of study: Risk factors Limits: Humans Country/Region as subject: Asia Language: English Journal: Infectio Journal subject: Communicable Diseases Year: 2020 Type: Article Affiliation country: India Institution/Affiliation country: A. J. Institute of Medical Sciences and Research Centre/IN / Employees State Insurance Corporation Medical College and Post Graduate Institute of Medical Science and Research/IN / HLL Biotech Ltd/IN / St. John's Medical College Hospital/IN

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Full text: Available Index: LILACS (Americas) Main subject: Carbapenems / Acinetobacter baumannii Type of study: Risk factors Limits: Humans Country/Region as subject: Asia Language: English Journal: Infectio Journal subject: Communicable Diseases Year: 2020 Type: Article Affiliation country: India Institution/Affiliation country: A. J. Institute of Medical Sciences and Research Centre/IN / Employees State Insurance Corporation Medical College and Post Graduate Institute of Medical Science and Research/IN / HLL Biotech Ltd/IN / St. John's Medical College Hospital/IN