Your browser doesn't support javascript.
loading
Effect of focal adhesion kinase inhibition on osteoblastic cells grown on titanium with different topographies
LOPES, Helena Bacha; SOUZA, Alann Thaffarell Portilho; FREITAS, Gileade Pereira; ELIAS, Carlos Nelson; ROSA, Adalberto Luiz; BELOTI, Marcio Mateus.
  • LOPES, Helena Bacha; Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Bone Research Laboratory. Ribeirão Preto. BR
  • SOUZA, Alann Thaffarell Portilho; Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Bone Research Laboratory. Ribeirão Preto. BR
  • FREITAS, Gileade Pereira; Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Bone Research Laboratory. Ribeirão Preto. BR
  • ELIAS, Carlos Nelson; Instituto Militar de Engenharia. Laboratório de Biomateriais. Rio de Janeiro. BR
  • ROSA, Adalberto Luiz; Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Bone Research Laboratory. Ribeirão Preto. BR
  • BELOTI, Marcio Mateus; Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto. Bone Research Laboratory. Ribeirão Preto. BR
J. appl. oral sci ; 28: e20190156, 2020. graf
Article in English | LILACS, BBO | ID: biblio-1090765
ABSTRACT
Abstract Objective The present study aimed to investigate the participation of focal adhesion kinases (FAK) in interactions between osteoblastic cells and titanium (Ti) surfaces with three different topographies, namely, untreated (US), microstructured (MS), and nanostructured (NS). Methodology Osteoblasts harvested from the calvarial bones of 3-day-old rats were cultured on US, MS and NS discs in the presence of PF-573228 (FAK inhibitor) to evaluate osteoblastic differentiation. After 24 h, we evaluated osteoblast morphology and vinculin expression, and on day 10, the following parameters gene expression of osteoblastic markers and integrin signaling components, FAK protein expression and alkaline phosphatase (ALP) activity. A smooth surface, porosities at the microscale level, and nanocavities were observed in US, MS, and NS, respectively. Results FAK inhibition decreased the number of filopodia in cells grown on US and MS compared with that in NS. FAK inhibition decreased the gene expression of Alp, bone sialoprotein, osteocalcin, and ALP activity in cells grown on all evaluated surfaces. FAK inhibition did not affect the gene expression of Fak, integrin alpha 1 ( Itga1 ) and integrin beta 1 ( Itgb1 ) in cells grown on MS, increased the gene expression of Fak in cells grown on NS, and increased the gene expression of Itga1 and Itgb1 in cells grown on US and NS. Moreover, FAK protein expression decreased in cells cultured on US but increased in cells cultured on MS and NS after FAK inhibition; no difference in the expression of vinculin was observed among cells grown on all surfaces. Conclusions Our data demonstrate the relevance of FAK in the interactions between osteoblastic cells and Ti surfaces regardless of surface topography. Nanotopography positively regulated FAK expression and integrin signaling pathway components during osteoblast differentiation. In this context, the development of Ti surfaces with the ability to upregulate FAK activity could positively impact the process of implant osseointegration.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Osteoblasts / Sulfones / Titanium / Quinolones / Focal Adhesion Protein-Tyrosine Kinases Limits: Animals Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Militar de Engenharia/BR / Universidade de São Paulo/BR

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Osteoblasts / Sulfones / Titanium / Quinolones / Focal Adhesion Protein-Tyrosine Kinases Limits: Animals Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Militar de Engenharia/BR / Universidade de São Paulo/BR