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High incidence of herpes simplex virus-1 in cord blood and placenta infection of women in southern Brazil / Alta incidência do vírus herpes simplex 1 em sangue de cordão e infecção na placenta de mulheres no sul do Brasil
Avila, Emiliana Claro; Finger-Jardim, Fabiana; Center for Obstetrics and GynecologyGonçalves, Carla Vitola; Hora, Vanusa Pousada da; Soares, Marcelo Alves; Martínez, Ana Maria Barral de.
  • Avila, Emiliana Claro; Universidade Federal do Rio Grande. School of Medicine. Molecular Biology Laboratory. Rio Grande. BR
  • Finger-Jardim, Fabiana; Universidade Federal do Rio Grande. School of Medicine. Molecular Biology Laboratory. Rio Grande. BR
  • Center for Obstetrics and GynecologyGonçalves, Carla Vitola; Universidade Federal do Rio Grande. School of Medicine. Center for Obstetrics and GynecologyGonçalves, Carla Vitola. Rio Grande. BR
  • Hora, Vanusa Pousada da; Universidade Federal do Rio Grande. School of Medicine. Molecular Biology Laboratory. Rio Grande. BR
  • Soares, Marcelo Alves; Instituto Nacional do Câncer. Oncovirology Program. Rio de Janeiro. BR
  • Martínez, Ana Maria Barral de; Universidade Federal do Rio Grande. School of Medicine. Molecular Biology Laboratory. Rio Grande. BR
Rev. bras. ginecol. obstet ; 42(1): 5-11, Jan. 2020. tab
Article in English | LILACS | ID: biblio-1092632
Responsible library: BR1.1
ABSTRACT
Abstract Objective Estimate the prevalence of human herpesvirus type 1 HSV-1 DNA in placental samples, its incidence in umbilical cord blood of newborns and the associated risk factors. Methods Placental biopsies and umbilical cord blood were analyzed, totaling 480 samples, from asymptomatic parturients and their newborns at a University Hospital. Nested polymerase chain reaction (PCR) and gene sequencingwere used to identify the virus; odds ratio (OR) and relative risk (RR) were performed to compare risk factors associated with this condition. Results The prevalence of HSV-1 DNA in placental samples was 37.5%, and the incidence in cord blood was 27.5%. Hematogenous transplacental route was identified in 61.4% from HSV-1+ samples of umbilical cord blood paired with the placental tissue. No evidence of the virus was observed in the remaining 38.6% of placental tissues, suggesting an ascendant infection from the genital tract, without replication in the placental tissue, resulting in intra-amniotic infection and vertical transmission, seen by the virus in the cord blood. The lack of condom use increased the risk of finding HSV-1 in the placenta and umbilical cord blood. Conclusion The occurrence of HSV-1 DNA in the placenta and in cord blood found suggests vertical transmission from asymptomatic pregnant women to the fetus.
RESUMO
Resumo Objetivo Estimar a prevalência do DNA do vírus herpes humano 1 (HSV-1) em amostras de placenta, sua incidência no sangue do cordão umbilical de recém-nascidos e fatores de risco associados. Métodos Biópsias de placenta e de sangue de cordão umbilical foram analisadas, totalizando 480 amostras de parturientes assintomáticas e seus recém-nascidos emum hospital universitário. Reação de cadeia de polimerase (RCP) nested e sequenciamento gênico foram usados para identificar o vírus; odds ratio (OR) e risco relativo (RR) foram realizados para comparar os fatores de risco associados à essa condição. Resultados A prevalência do DNA do HSV-1 em amostras de placenta foi de 37,5%, e a incidência no sangue do cordão foi de 27,5%. A via transplacentária hematogênica foi identificada em 61,4% das amostras de HSV-1+do sangue do cordão umbilical, pareadas com o tecido placentário. Nenhuma evidência do vírus foi observada nos restantes 38,6% dos tecidos placentários, sugerindo uma infecção ascendente do trato genital. A falta de uso do preservativo aumentou o risco de encontrar o HSV-1 na placenta e no sangue do cordão umbilical. Conclusão A ocorrência de DNA do HSV-1 na placenta e no sangue do cordão umbilical sugere uma transmissão vertical de gestantes assintomáticas para o feto.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Pregnancy Complications, Infectious / Herpesvirus 1, Human / Herpes Simplex Type of study: Etiology study / Incidence study / Prognostic study / Risk factors / Screening study Limits: Adult / Female / Humans / Infant, Newborn / Pregnancy Country/Region as subject: South America / Brazil Language: English Journal: Rev. bras. ginecol. obstet Journal subject: Gynecology / Obstetrics Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Nacional do Câncer/BR / Universidade Federal do Rio Grande/BR

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Full text: Available Index: LILACS (Americas) Main subject: Pregnancy Complications, Infectious / Herpesvirus 1, Human / Herpes Simplex Type of study: Etiology study / Incidence study / Prognostic study / Risk factors / Screening study Limits: Adult / Female / Humans / Infant, Newborn / Pregnancy Country/Region as subject: South America / Brazil Language: English Journal: Rev. bras. ginecol. obstet Journal subject: Gynecology / Obstetrics Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Nacional do Câncer/BR / Universidade Federal do Rio Grande/BR