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Wolbachia spp. interfere na detecção molecular de Ehrlichia canis e Anaplasma platys em cães com microfilaremia / Wolbachia spp. interferes with molecular detection of Ehrlichia canis and Anaplasma platys in microfilaremic dogs
Xavier, M. S; Fonseca, C. N; Ferreira, R. F; Castro, T. X; Neves, F. P. G; Barbosa, A. V; Cerqueira, A. M. F; Almosny, N. R. P.
  • Xavier, M. S; Universidade Federal Fluminense. Faculdade de Veterinária. Niterói. BR
  • Fonseca, C. N; Universidade Federal Fluminense. Faculdade de Veterinária. Niterói. BR
  • Ferreira, R. F; Universidade Federal Fluminense. Faculdade de Veterinária. Niterói. BR
  • Castro, T. X; Universidade Federal Fluminense. Instituto Biomédico. Niterói. BR
  • Neves, F. P. G; Universidade Federal Fluminense. Instituto Biomédico. Niterói. BR
  • Barbosa, A. V; Universidade Federal Fluminense. Instituto Biomédico. Niterói. BR
  • Cerqueira, A. M. F; Universidade Federal Fluminense. Instituto Biomédico. Niterói. BR
  • Almosny, N. R. P; Universidade Federal Fluminense. Faculdade de Veterinária. Niterói. BR
Arq. bras. med. vet. zootec ; 67(4): 1197-1200, July-Aug. 2015. tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1095962
ABSTRACT
This report aimed to study the interference in molecular testing for Ehrlichia canis and Anaplasma platys in blood of 155 dogs from the coastal region of Rio de Janeiro. Five Anaplasmataceae positive samples but negative for E. canis and A. platys, from microfilaremic animals, were chosen for sequencing. These sequences, when compared to Gen et Bank database, showed 88% to 100% similarity with Wolbachia spp. denoting an interference in the detection of DNA from other members of Anaplasmataceae, possibly due to a high concentration of Wolbachia spp. DNA.(AU)
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Full text: Available Index: LILACS (Americas) Main subject: Wolbachia / Ehrlichia canis / Anaplasma / Microfilariae Type of study: Diagnostic study Limits: Animals Language: Portuguese Journal: Arq. bras. med. vet. zootec Year: 2015 Type: Article Institution/Affiliation country: Universidade Federal Fluminense/BR

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Full text: Available Index: LILACS (Americas) Main subject: Wolbachia / Ehrlichia canis / Anaplasma / Microfilariae Type of study: Diagnostic study Limits: Animals Language: Portuguese Journal: Arq. bras. med. vet. zootec Year: 2015 Type: Article Institution/Affiliation country: Universidade Federal Fluminense/BR