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AIF, PARP-1 and MicroRNA-9 expression in cerebral ischemia associated to alcoholism experimental model / Expresión de AIF, PARP-1 y MicroRNA-9 de isquemia cerebral asociado a un modelo experimental de alcoholismo
Abrahão, Dayana Pousa Siqueira; Lizarte Neto, Fermino Sanches; Rodrigues, Andressa Romualdo; Cirino, Múcio Luiz de Assis; Silva, Jairo Pinheiro da; Sarraipo, Vagner Schiavoni; Carvalho, Camila Albuquerque Melo de; Tazima, Maria de Fátima Galli Sorita; Carlotti-Jr, Carlos Gilberto; Colli, Benedicto Oscar; Tirapelli, Luís Fernando; Tirapelli, Daniela Pretti da Cunha.
  • Abrahão, Dayana Pousa Siqueira; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Lizarte Neto, Fermino Sanches; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Rodrigues, Andressa Romualdo; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Cirino, Múcio Luiz de Assis; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Silva, Jairo Pinheiro da; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Sarraipo, Vagner Schiavoni; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Carvalho, Camila Albuquerque Melo de; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Tazima, Maria de Fátima Galli Sorita; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Carlotti-Jr, Carlos Gilberto; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Colli, Benedicto Oscar; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Tirapelli, Luís Fernando; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
  • Tirapelli, Daniela Pretti da Cunha; Medicine School of Ribeirão Preto. Department of Surgery and Anatomy. Ribeirão Preto. BR
Int. j. morphol ; 38(3): 616-621, June 2020. graf
Article in English | LILACS | ID: biblio-1098296
Responsible library: CL1.1
ABSTRACT
The chronic consumption of alcohol causes a worsening of the events that follow the cerebral ischemia. These events are regulated through the expression of several genes and microRNAs. The aimof this work was To analyze and describe the expression profile of PARP and AIF and miRNA-9 proteins in rats submitted to focal cerebral ischemia, associated or not with chronic alcoholism model.

Methods:

Twenty adult Wistar rats, subdivided into control; ischemic; alcoholic and ischemic / alcoholized for immunohistochemical analysis and miRNA-9 gene expression.

Results:

There was a reduction in the protein expression of PARP-1 and a positive marking for AIF in the ischemic / alcoholized group. The miRNA-9 did not obtain significant expression. The association of ischemia with chronic alcohol use promoted a tendency to low expression of miRNA-9, low expression of PARP-1 and high expression of AIF, indicating an interference in the protective effect of miRNA-9 be observed in the other groups.
RESUMEN
El consumo crónico de alcohol provoca un empeoramiento de los eventos que siguen a la isquemia cerebral. Estos eventos están regulados a través de la expresión de varios genes y microRNA. El objetivo de este trabajo fue analizar y describir el perfil de expresión de las proteínas PARP y AIF y microRNA-9 en ratas sometidas a isquemia cerebral focal, asociadas o no, con el modelo de alcoholismo crónico. Veinte ratas Wistar adultas se dividieron en grupo control, isquémico alcohólico, e isquémico / alcoholizado para análisis inmunohistoquímico y expresión de genes microRNA-9.

Resultados:

Hubo una reducción en la expresión de proteínas de PARP-1 y un marcado positivo para AIF en el grupo isquémico / alcoholizado. No se observó una expresión significativa en el microRNA-9. La asociación de la isquemia con el consumo crónico de alcohol promovió una tendencia a la baja expresión de microRNA-9, baja expresión de PARP1 y alta expresión de AIF, lo que indica una interferencia en el efecto protector de microRNA-9 en los otros grupos.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Brain Ischemia / Alcoholism Type of study: Prognostic study / Risk factors Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Medicine School of Ribeirão Preto/BR

Full text: Available Index: LILACS (Americas) Main subject: Brain Ischemia / Alcoholism Type of study: Prognostic study / Risk factors Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Medicine School of Ribeirão Preto/BR