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Protective effects of honey, Apis mellifera meda skorikov, on ischemia-reperfusion induced muscle injury / Efectos protectores de la miel, Apis mellifera meda skorikov, sobre la lesión muscular inducida por isquemia-reperfusión
Gholami, Mohammad Reza; Abbaszadeh, Abolfazl; Anbari, Khatereh; Khaksarian, Mojtaba; Shabooni, Fatemeh; Khayat, Zahra Khanipour; Khorramabadi, Reza Mohammadrezaei; Gharravi, Anneh Mohammad.
  • Gholami, Mohammad Reza; Kermanshah University of Medical Sciences. Medical Biology Research Center. Kermanshah. IR
  • Abbaszadeh, Abolfazl; Lorestan University of Medical Sciences. Razi Herbal Medicines Research Center. Khorramabad. IR
  • Anbari, Khatereh; Lorestan University of Medical Sciences. Department of Social Medicine. Khorramabad. IR
  • Khaksarian, Mojtaba; Lorestan University of Medical Sciences. Razi Herbal Medicines Research Center. Khorramabad. IR
  • Shabooni, Fatemeh; Lorestan University of Medical Sciences. Khorramabad. IR
  • Khayat, Zahra Khanipour; Lorestan University of Medical Sciences. Razi Herbal Medicines Research Center. Khorramabad. IR
  • Khorramabadi, Reza Mohammadrezaei; Lorestan University of Medical Sciences. Khorramabad. IR
  • Gharravi, Anneh Mohammad; Shahroud University of Medical Sciences. Stem Cells and Tissue Engineering Research Center. Shahroud. IR
Int. j. morphol ; 38(3): 804-810, June 2020. tab, graf
Article in English | LILACS | ID: biblio-1098323
Responsible library: CL1.1
ABSTRACT
Honey is a natural antioxidant that its protective effects have been proven against ischemia-reperfusion (IR) injury. The aim of this study was to evaluate the ameliorative effect of Persian Honey, Apis mellifera meda skorikov, on gastrocnemius muscle IR injury. Eighty adult male Sprague-Dawley rats weighing 250-300 g were used. They were divided into ten groups (N=8 per group). The ischemia was conducted with a silk suture 6-0 using the slipknot technique. All groups were rendered in ischemic for 3 h, and reperfused for various times of 3 days (3-day reperfusion), 7 days (7-day reperfusion), 14 days (14-day reperfusion), and 28 days (28-day reperfusion). Half of the groups had experimental honey (5 %) treatment immediately after ischemia. After reperfusion, the rats, based on the grouping, were killed with high doses of anesthetic, and the gastrocnemius muscles were removed and fixed. After the tissue processing, the evaluation of edema and mast cells infiltration was performed with hematoxylin-eosin and toluidine blue staining, respectively. TNF-α was detected with immunohistochemistry method. The amount of TNF-α as an index of acute inflammatory except the 3rd day significantly decreased on the other day of reperfusion (7th, 147th and 287th days). The mast cells infiltration was significantly decreased on 77th and 147th days. The tissue edema was decreased significantly in honey administrated group in the comparison with placebo groups. Honey administration can reduce damage caused by ischemia-reperfusion in the rat gastrocnemius muscle.
RESUMEN
La miel es un antioxidante natural; sus efectos protectores han sido probados contra la lesión por isquemiareperfusión (IR). El objetivo de este estudio fue evaluar el efecto de mejora de la miel persa Apis mellifera meda skorikov, en la lesión por IR del músculo gastrocnemio. Se utilizaron 80 ratas Sprague-Dawley macho adultas con un peso entre 250 y 300 g divididas en diez grupos (N = 8 por grupo). La isquemia se realizó con una sutura de seda 6-0 utilizando la técnica slipknot permaneciendo isquémicos durante 3 h. La reperfusión se realizó durante varios tiempos de 3 días, 7 días (reperfusión de 7 días), 14 días (reperfusión de 14 días) y 28 días (28 días reperfusión). La mitad de los grupos recibió tratamiento experimental con miel (5 %) inmediatamente después de la isquemia. Después de la reperfusión, las ratas, fueron sacrificadas con altas dosis de anestésico, y los músculos gastrocnemios fueron removidos y fijados. Después de procesar el tejido, se realizó la evaluación del edema y la infiltración de mastocitos se realizó con tinción de hematoxilina-eosina y azul de toluidina, respectivamente. TNF-α se detectó con el método de inmunohistoquímica. La cantidad de TNF-α como índice de inflamación inflamatoria aguda, excepto en el tercer día, disminuyó significativamente al día siguiente de la reperfusión (7, 14 y 28 días). La infiltración de mastocitos disminuyó significativamente a los 7 y 14 días. El edema tisular disminuyó significativamente en el grupo administrado con miel en comparación con los grupos placebo. El tratamiento con miel puede reducir el daño causado por la isquemia-reperfusión en el músculo gastrocnemio de la rata.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Reperfusion Injury / Apis mellifica / Muscle, Skeletal / Honey Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: Iran Institution/Affiliation country: Kermanshah University of Medical Sciences/IR / Lorestan University of Medical Sciences/IR / Shahroud University of Medical Sciences/IR

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Full text: Available Index: LILACS (Americas) Main subject: Reperfusion Injury / Apis mellifica / Muscle, Skeletal / Honey Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: Iran Institution/Affiliation country: Kermanshah University of Medical Sciences/IR / Lorestan University of Medical Sciences/IR / Shahroud University of Medical Sciences/IR