Remote ischemic conditioning protects against testicular ischemia∕reperfusion injury in rats
Acta cir. bras
;
35(2): e202000203, 2020. graf
Article
in English
| LILACS
| ID: biblio-1100882
ABSTRACT
Abstract Purpose To evaluate the effect of remote ischemic conditioning associated to N-acetylcysteine (NAC) on testicular ischemia∕reperfusion (I∕R) injury in rats. Methods Twenty-five adult male Wistar rats were randomly distributed into five experimental groups (n=5), as follows Sham, I∕R, Perconditioning (PER), NAC and PER+NAC. Two-hour ischemia was induced by rotating the left testis 720° to clockwise direction, followed by 4 hours of reperfusion. Perconditioning was performed by three I/R cycles of 10 min each on the left limb, 30 min before reperfusion. N-acetylcysteine (150 mg∕kg) was administered 30 min before reperfusion. Results Statistical differences were observed in MDA levels between I/R group with all groups (p<0.01), in addition there was statistical difference between PER and Sham, and PER+ NAC groups (p<0.05) in plasma. Conclusions The protective effect of perconditioning isolated in the reduction of lipid peroxidation related to oxidative stress was demonstrated. However, when Perconditioning was associated with NAC, there was no protective effect against testicular injury of ischemia and reperfusion.
Full text:
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Index:
LILACS (Americas)
Main subject:
Acetylcysteine
/
Testis
/
Reperfusion Injury
/
Free Radical Scavengers
/
Oxidative Stress
/
Ischemic Preconditioning
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2020
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Centro Universitário do Estado do Pará/BR
/
UEPA/BR
/
Universidade do Estado do Pará/BR
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