Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 gene
Biol. Res
;
53: 10, 2020. tab, graf
Article
in English
| LILACS
| ID: biblio-1100916
ABSTRACT
BACKGROUND:
The aim of this study was to investigate the effect role and mechanism of miR-30b-3p on ovarian cancer cells biological function.METHODS:
The expression of miR-30b-3p was detected in ovarian cancer cell lines and normal ovarian epithelial cell line by qRT-PCR. Mir-30b-3p mimic was transfected into OVCAR3 cells. Cell-counting kit-8 (CCK-8) assay was conducted to explore the effect of mir-30b-3p on the OVCAR3 cells' proliferation. Cell cycle and apoptosis were detected by Flow cytometry. Cell invasion ability was detected by Transwell test. The regulation of putative target of miR-30b-3p was verified by double luciferase reporter assays and Western blot.RESULT:
We found that miR-30b-3p was downregulated in OVCAR3 cells. Overexpression of miR-30b-3p suppressed proliferation, promoted apoptosis, slowed cell cycle and inhibited migration and invasion of OVCAR3 cells. Bioinformatics analysis identified 3'-untranslated region (3'UTR) of Collagen triple helix repeat-containing 1 (CTHRC1) as the presumed binding site for miR-30b-3p. Detection of double luciferase reporter and Western-Blot result confirmed that CTHRC1 was the target gene of miR-30b-3p. Furthermore, E-cadherin, ß-cadherin and Vimentin protein expression level were changed after transfection of miR-30b-3p.CONCLUSION:
miR-30b-3p function as an anti-cancer gene. Overexpression of miR-30b-3p can inhibit the biological function of ovarian cancer cells. MiR-30b-3p targets CTHRC1 gene plays an important role in epithelial-mesenchymal transformation (EMT), and supports miR-30b-3p as a potential biological indicator for ovarian cancer in the future.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Ovarian Neoplasms
/
Gene Expression Regulation, Neoplastic
/
Extracellular Matrix Proteins
/
MicroRNAs
/
Epithelial-Mesenchymal Transition
Limits:
Female
/
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2020
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
The First Affiliated Hospital of Soochow University/CN
/
The First People's Hospital of Yancheng/CN
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