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Leishmaniose Tegumentar Americana: uma análise histopatológica e molecular em lesões de dermatites no estado de Pernambuco, Brasil / American Cutaneous Leishmaniasis: a histopathological and molecular analysis of dermatitis lesions in the State of Pernambuco, Brazil
Silva, Maria Auzenir de Anastácio e; Soares, Cynthia Regina Pedrosa; Melo, Fábio Lopes de; Rocha, Francisca Janaína Soares.
  • Silva, Maria Auzenir de Anastácio e; Universidade Federal de Pernambuco - UFPE. Recife. BR
  • Soares, Cynthia Regina Pedrosa; Universidade Federal de Pernambuco - UFPE. Recife. BR
  • Melo, Fábio Lopes de; Fundação Oswaldo Cruz - FIOCRUZ. Recife. BR
  • Rocha, Francisca Janaína Soares; Universidade Federal de Pernambuco - UFPE. Recife. BR
Rev. bras. anal. clin ; 51(4): 315-321, 2019/12/30. ilus ilus, tab
Article in Portuguese | LILACS | ID: biblio-1104009
RESUMO

Objetivo:

Realizar uma análise histopatológica e molecular em biópsia de pele entre as lesões de dermatites de pacientes com suspeita de Leishmaniose Tegumentar Americana (LTA) no hospital de referência do estado de Pernambuco entre o período de 2016 e 2017.

Métodos:

Trata-se de um estudo descritivo observacional, no qual todos os pacientes com lesões clinicamente sugestivas para LTA incluídos no estudo foram submetidos à coleta de biópsia de pele das lesões, as quais foram analisadas pela técnica histopatológica e PCR (Reação em Cadeia de Polimerase).

Resultados:

Foram analisadas 24 amostras de biópsia de pele de pacientes com suspeita clínica de LTA, por testes histopatológicos e confirmação pela PCR. As amostras foram caracterizadas pela busca do DNA de Leishmania braziliensis através da PCR. Das 24 amostras estudadas, em nenhuma foi encontrado DNA de L. braziliensis. Apenas em um caso foi detectada presença de amastigotas de Leishmania pela técnica histopatológica. Outros achados microscópicos observados foram dermatite granulomatosa (33,33%), úlcera crônica (20,83%), carcinoma basocelular (16,66%), Leishmaniose, dermatite plasmocitária e inflamação granulomatosa (8,33%) e Hanseníase (4,16%).

Conclusão:

O diagnóstico histopatológico detectou um caso de LTA, porém, a PCR não encontrou DNA do parasito. A análise histopatológica mostrou que as lesões dermatotrópicas dos pacientes são oriundas principalmente de úlceras, tumores de pele e hanseníase.
ABSTRACT

Objective:

Accomplish a histopathological and molecular analysis in skin biopsy between the dermatitis lesions of patients with suspected American Cutaneous Leishmaniasis (ATL) at the Hospital of Reference of the State of Pernambuco between the period of 2016 and 2017.

Methods:

This is a descriptive, observational study in which all patients with clinically suggestive lesions for ATL included in the study were submitted to skin biopsy of the lesions and analyzed by the histopathological technique and PCR (Polymerase Chain Reaction).

Results:

Were analyzed 24 skin biopsy samples from patients with clinical suspicion of ATL, by histopathological tests and confirmation by PCR. Samples were characterized by the search of Leishmania braziliensis DNA through PCR. Of the 24 samples studied, no DNA of L. braziliensis was found. Only in one case was detected presence of Leishmania amastigotes by histopathological technique. Other microscopic findings were granulomatous dermatitis (33.33%), chronic ulcer (20.83%), basal cell carcinoma (16.66%), Leishmaniasis, plasmacytoma dermatitis and granulomatous inflammation (8.33%) and leprosy, 16%).

Conclusion:

The histopathological diagnosis detected a case of ATL, however, the PCR did not find DNA of the parasite. The histopathological analysis showed that the dermatotropic lesions of the patients come mainly from ulcers, skin tumors and leprosy.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Leishmania braziliensis / Polymerase Chain Reaction / Leishmaniasis, Cutaneous / Dermatitis Type of study: Observational study Limits: Adult / Aged / Female / Humans / Male Country/Region as subject: South America / Brazil Language: Portuguese Journal: Rev. bras. anal. clin Journal subject: Pathology Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz - FIOCRUZ/BR / Universidade Federal de Pernambuco - UFPE/BR

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Full text: Available Index: LILACS (Americas) Main subject: Leishmania braziliensis / Polymerase Chain Reaction / Leishmaniasis, Cutaneous / Dermatitis Type of study: Observational study Limits: Adult / Aged / Female / Humans / Male Country/Region as subject: South America / Brazil Language: Portuguese Journal: Rev. bras. anal. clin Journal subject: Pathology Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz - FIOCRUZ/BR / Universidade Federal de Pernambuco - UFPE/BR