Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota)
Acta amaz
;
50(1): 61-67, jan. - mar. 2020.
Article
in English
| LILACS
| ID: biblio-1118663
ABSTRACT
The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed. (AU)
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Index:
LILACS (Americas)
Main subject:
Spores
/
Amazonian Ecosystem
/
Culture Media
/
Fungi
Language:
English
Journal:
Acta amaz
Journal subject:
Science
Year:
2020
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Nacional de Pesquisas da Amazônia/BR
/
Universidade Federal do Amazonas/BR
/
Universidade Federal do Oeste do Pará/BR
/
Universidade Federal do Rio Grande do Norte/BR
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