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Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species
Fernandes-Rocha, Lucas; Ribeiro-Paula, Natália; Gonçalves-Nazareno, Alison; Carvalho, Dulcinéia de.
  • Fernandes-Rocha, Lucas; São Paulo State University. Department of Forest Science. Botucatu. BR
  • Ribeiro-Paula, Natália; Federal University of Lavras. Laboratory of Tree Genetic Conservation. Department of Forest Sciences. Lavras. BR
  • Gonçalves-Nazareno, Alison; Federal University of Minas Gerais. Department of Biology. Belo Horizonte. BR
  • Carvalho, Dulcinéia de; Federal University of Lavras. Laboratory of Tree Genetic Conservation. Department of Forest Sciences. Lavras. BR
Biol. Res ; 53: 30, 2020. tab
Article in English | LILACS | ID: biblio-1124215
ABSTRACT

BACKGROUND:

We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species.

RESULTS:

Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed ( HO ) and expected ( HE ) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively.

CONCLUSIONS:

These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy.
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Full text: Available Index: LILACS (Americas) Main subject: Endangered Species / Microsatellite Repeats / Asteraceae Country/Region as subject: South America / Brazil Language: English Journal: Biol. Res Journal subject: Biology Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Lavras/BR / Federal University of Minas Gerais/BR / São Paulo State University/BR

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Full text: Available Index: LILACS (Americas) Main subject: Endangered Species / Microsatellite Repeats / Asteraceae Country/Region as subject: South America / Brazil Language: English Journal: Biol. Res Journal subject: Biology Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Lavras/BR / Federal University of Minas Gerais/BR / São Paulo State University/BR