Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species
Biol. Res
;
53: 30, 2020. tab
Article
in English
| LILACS
| ID: biblio-1124215
ABSTRACT
BACKGROUND:
We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species.RESULTS:
Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed ( HO ) and expected ( HE ) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively.CONCLUSIONS:
These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy.
Full text:
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Index:
LILACS (Americas)
Main subject:
Endangered Species
/
Microsatellite Repeats
/
Asteraceae
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2020
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Lavras/BR
/
Federal University of Minas Gerais/BR
/
São Paulo State University/BR
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