Actividad antitumoral de biopolímeros aislados de Vibrio sp. en cáncer de mama inducido en ratas / Antitumor activity of vibrio sp. isolated biopolymers in induced breast cancer in rats
Rev. peru. med. exp. salud publica
;
37(2): 246-252, abr.-jun. 2020. tab, graf
Article
in Spanish
| LILACS
| ID: biblio-1127148
RESUMEN
RESUMEN Objetivo:
Evaluar la actividad antitumoral del extracto crudo de biopolímeros aislados de la bacteria marina Vibrio sp. en cáncer de mama inducido por N-Methyl-N-nitrosourea (MNU) en ratas. Materiales ymétodos:
Se cultivó la bacteria marina Vibrio sp. durante siete días, luego se filtró, precipitó y concentró el sobrenadante crudo. Se administró una dosis única de MNU 50 mg/kg a 39 ratas Holtzman y fueron tratadas diariamente durante nueve semanas por vía oral G1 (n=13) suero fisiológico 0,1 mL/100g; G2 (n=13) extracto crudo de biopolímeros de Vibrio sp. 20 mg/kg; G3 (n=13) tamoxifeno 100 mg/kg. El G4 (n=11) solo recibió suero fisiológico 0,1 mL/100g. Se valoró semanalmente el peso corporal y la aparición de tumores mamarios identificados mediante palpación; así como el examen histopatológico al final del tratamiento.Resultados:
El 77% de las ratas del grupo G1 desarrollaron tumores a partir de la séptima semana en un promedio de 2,2 tumores por cada animal; en contraste al grupo tratado con el extracto crudo de biopolímeros y tamoxifeno; donde solo una rata (8%) en cada grupo desarrolló tumores y posterior a la semana nueve de la inducción (p=0,001). Los resultados histopatológicos sostienen que todos los tumores extirpados corresponden a adenocarcinoma ductal de mama con distintos patrones sólido, papilar y quístico. Asimismo, se evidenciaron focos necróticos en el 30% de los tumores del grupo G1.Conclusión:
El extracto crudo de biopolímeros aislados de Vibrio sp. presentan efecto antitumoral en cáncer de mama inducido en ratas.ABSTRACT
ABSTRACT Objective:
To evaluate the antitumor activity of the raw extract from biopolymers isolated from the Vibrio sp. marine bacteria in breast cancer induced by N-Methyl-N-nitrosourea (MNU) in rats. Materials andmethods:
The Vibrio sp. marine bacteria was cultured for seven days, then the raw supernatant was filtered, precipitated and concentrated. MNU was administered in a single dose of 50 mg/kg to 39 Holtzman rats and were daily treated for 9 weeks orally G1 (n = 13) 0.1 mL/100 g of saline solution; G2 (n = 13) 20 mg/kg of raw extract from Vibrio sp. biopolymers; G3 (n = 13) 100 mg/kg of tamoxifen; G4 (n = 11) received no MNU and only 0.1 mL/100 g of saline solution. Body weight and the appearance of breast tumors identified by palpation were assessed weekly, as well as histopathological examination at the end of treatment.Results:
Seventy-seven percent of the rats in the G1 group developed tumors from week 7 onwards in an average of 2.2 tumors per animal; in contrast to the group treated with the raw biopolymer extract and tamoxifen; where only one rat (8%) in each group developed tumors after week nine of induction (p = 0.001). The histopathological results support that all the removed tumors correspond to breast ductal adenocarcinoma with different patterns solid, papillary and cystic. Likewise, necrotic foci were evidenced in 30% of the tumors of the G1 group.Conclusion:
The raw extract of biopolymers isolated from Vibrio sp. present antitumor effect in breast cancer induced in rats.
Full text:
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Index:
LILACS (Americas)
Main subject:
Rats
/
Vibrio
/
Biopolymers
/
Breast Neoplasms
/
Mammary Neoplasms, Experimental
/
Antineoplastic Agents
Limits:
Animals
Language:
Spanish
Journal:
Rev. peru. med. exp. salud publica
Journal subject:
Public Health
Year:
2020
Type:
Article
Affiliation country:
Peru
Institution/Affiliation country:
Universidad Nacional Mayor de San Marcos/PE
/
Universidad Nacional Tecnológica de Lima Sur (UNTELS)/PE
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