Histological analysis of the intestinal wall of newborn rats submitted to hypoxia and reoxygenation to evaluate the protective effect of N-Acetylcysteine
Acta cir. bras
;
35(4): e202000401, 2020. tab, graf
Article
in English
| LILACS
| ID: biblio-1130631
ABSTRACT
Abstract Purpose To evaluate the effect of N-Acetylcysteine (NAC) in newborn rats submitted to hypoxia and reoxygenation (H/R) conditions in an experimental model of necrotizing enterocolitis. Methods Eight pregnant rats and their 70 cubs were used (5 groups) and exposed to H/R conditions and received NAC at different times. The animals in the H/R groups were placed in a gas chamber (100% CO2) for 10 minutes and then reoxygenated for 10 minutes (100% O2), twice a day for the first three days of life, with a six-hour span between events. On the third day of life, the animals were anesthetized, laparotomized and the intestines were resected. Results The H/R and NAC groups showed changes in the intestinal wall in relation to the number, height and width of the villi when compared to the control group (p<0.0001), but with better preservation of structures in the NAC group. There were no differences between groups regarding the number (%) of mitoses. Conclusion The administration of NAC decreased the lesions in the intestinal wall of rats submitted to H/R, therefore suggesting that this drug can be used to prevent the development of necrotizing enterocolitis in newborns.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Acetylcysteine
/
Protective Agents
/
Enterocolitis, Necrotizing
/
Ileum
/
Hypoxia
Type of study:
Prognostic study
Limits:
Animals
/
Pregnancy
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2020
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
UNIFESP/BR
/
Universidade Federal de São Paulo/BR
/
Universidade de São Paulo/BR
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