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Comparative study between fructose 1-6 bisphosphate and histidine-tryptophan-ketoglutarate in liver preservation in rats submitted to total cold ischemia
Smecellato, Fernanda Bombonato; Marsilli, Lucas Ricardo Benfatti; Nakamura, Julia Eico; Jordani, Maria Cecília; Department of Surgery and AnatomyÉvora, Paulo Roberto Barbosa; Department of Surgery and AnatomyCastro-e-Silva, Orlando.
  • Smecellato, Fernanda Bombonato; FAMEMA. BR
  • Marsilli, Lucas Ricardo Benfatti; FAMEMA. BR
  • Nakamura, Julia Eico; FAMEMA. BR
  • Jordani, Maria Cecília; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirao Preto. BR
  • Department of Surgery and AnatomyÉvora, Paulo Roberto Barbosa; USP. FMRP. Department of Surgery and AnatomyÉvora, Paulo Roberto Barbosa. Ribeirao Preto. BR
  • Department of Surgery and AnatomyCastro-e-Silva, Orlando; USP. FMRP. Department of Surgery and AnatomyCastro-e-Silva, Orlando. Ribeirao Preto. BR
Acta cir. bras ; 35(6): e202000603, 2020. graf
Article in English | LILACS | ID: biblio-1130651
ABSTRACT
Abstract Purpose To compare Fructose-1,6-Bisphosphate (FBP) to Histidine-Tryptophan-Ketoglutarate (HTK) in liver preservation at cold ischemia. Methods Male rats (Sprague-Dawley 280-340g) divided into three groups (n=7) Control; Fructose-1,6-bisphosphate (FBP); Histidine-Tryptophan-Ketoglutarate (HTK). Animals underwent laparotomy-thoracotomy for perfusion of livers with saline. Livers were removed and deposited into solutions. Mitochondria were isolated to determine State 3 (S3), State 4 (S4), Respiratory Control Ratio (RCR) and Swelling (S). Liver enzymes (AST, ALT, LDH) were determined in solution. At tissue, Malondialdehyde (MDA) and Nitrate (NOx) were determined. All parameters were analyzed at 0.6 and 24 hours of hypothermic preservation. Statistics analysis were made by Mann-Whitney test (p<0.05). Results Regarding ALT, there was a difference between FBP-6h/HTK-6h, lower in HTK. Regarding AST, there was a significant difference between FBP-24h/HTK-24h, lower in FBP. Regarding NOx, there was a difference between 0h and 6h, as well as 0h and 24h for both solutions. Regarding S3, there was a significant difference in 24h compared to Control-0h for both solutions, and a significant difference between FBP-6h/FBP-24h. Regarding S4, there was a difference between Control-0h/HTK-24h and FBP-24h/HTK-24h, higher in HTK. There was a difference between Control-0h/FBP-24h for Swelling, higher in FBP. Conclusion Fructose-1,6-Bisphosphate showed better performance at nitrate and aspartate aminotransferase compared to histidine-tryptophan-ketoglutarate.
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Full text: Available Index: LILACS (Americas) Main subject: Cold Ischemia Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: FAMEMA/BR / USP/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Cold Ischemia Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: FAMEMA/BR / USP/BR / Universidade de São Paulo/BR