Chronic alcoholism associated with diabetes induced apoptosis in the corpus cavernosum of rats
Acta cir. bras
;
35(8): e202000805, 2020. graf
Article
in English
| LILACS
| ID: biblio-1130667
ABSTRACT
Abstract Purpose To evaluate the effects of alcohol exposure and diabetes on apoptotic process in the corpus cavernosum. Methods Forty eight male Wistar rats were divided into four groups control, diabetic, alcoholic and diabetic-alcoholic. Samples of the corpus cavernosum were prepared to study protein expression of apoptotic genes (Caspases-3 and 9) by immunohistochemistry and Real-Time PCR. Results The immunoreactivity of Caspases-3 and -9 was diffuse and higher in the treated groups though there was no significant difference between the experimental groups, only when compared with the control group. An increase was observed in the gene expression of Caspases-9 in the diabetic and ethanol-diabetic groups when compared with control and ethanol groups. Conclusions The association of these factors (ethanol and diabetes) probably can affect the apoptosis mechanism in lesions of the cavernous tissue in the rat penis. Both gene and protein expression of Caspase-9 in diabetic and ethanol-diabetic groups suggest the involvement of the apoptosis cascade from this study model.
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Index:
LILACS (Americas)
Main subject:
Apoptosis
/
Alcoholism
Type of study:
Prognostic study
/
Risk factors
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2020
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
USP/BR
/
Universidade Estadual Paulista/BR
/
Universidade de Marília/BR
/
Universidade de São Paulo/BR
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