Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723
Biol. Res
;
53: 35, 2020. graf
Article
in English
| LILACS
| ID: biblio-1131881
ABSTRACT
BACKGROUND:
Spinal cord injury (SCI) is a severe central nervous system trauma. The present study aimed to evaluate the effect of HIF-1α on inflammation in spinal cord injury (SCI) to uncover the molecular mechanisms of anti-inflammation.RESULTS:
HIF-1α was reduced in SCI model rats and HIF-1α activation reduced TNF-α, IL-1ß, IL-6 and IL-18 levels in SCI model rats. Meanwhile, Circ 0001723 expression was down-regulated and miR-380-3p expression was up-regulated in SCI model rats. In vitro model, down-regulation of Circ 0001723 promoted TNF-α, IL-1ß, IL-6 and IL-18 levels, compared with control negative group. However, over-expression of Circ 0001723 reduced TNF-α, IL-1ß, IL-6 and IL-18 levels in vitro model. Down-regulation of Circ 0001723 suppressed HIF-1α protein expressions and induced NLRP3 and Caspase-1 protein expressions in vitro model by up-regulation of miR-380-3p. Next, inactivation of HIF-1α reduced the pro-inflammation effects of Circ 0001723 in vitro model. Then, si-NLRP3 also inhibited the pro-inflammation effects of Circ 0001723 in vitro model via promotion of autophagy.CONCLUSIONS:
We concluded that HIF-1α reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Spinal Cord Injuries
/
MicroRNAs
/
Hypoxia-Inducible Factor 1, alpha Subunit
/
NLR Family, Pyrin Domain-Containing 3 Protein
/
RNA, Circular
/
Inflammation
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2020
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
The First Affiliated Hospital of Zhejiang University/CN
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