Brazilian Green Propolis: Chemical Composition of Essential Oil and Their In Vitro Antioxidant, Antibacterial and Antiproliferative Activities
Braz. arch. biol. technol
;
63: e20190408, 2020. tab
Article
in English
| LILACS
| ID: biblio-1132168
ABSTRACT
Abstract Propolis is a resinous substance collected and processed by Apis mellifera from parts of plants, buds and exudates. In Minas Gerais (MG) state, Brazil, green propolis is produced from the collection of resinous substance found in shoot apices of Baccharis dracunculifolia. This paper aims to investigate the chemical composition and in vitro antioxidant, anti-Helicobacter pylori, antimycobacterial and antiproliferative activities of essential oil (EO) from Brazilian green propolis (BGP-EO). The oil showed high antibacterial activity against H. pylori (MIC = 6.25 µg/mL), Mycobacterium avium (MIC = 62.5 µg/mL) and M. tuberculosis (MIC = 64 µg/mL). Its antioxidant activity was evaluated in vitro by both DPPH (IC50 = 23.48 µg/mL) and ABTS (IC50 = 32.18 µg/mL) methods. The antiproliferative activity in normal (GM07492A, lung fibroblasts) and tumor cell lines (MCF-7, HeLa and M059J) was analyzed by the XTT assay. BGP-EO showed inhibition of normal cell growth at 68.93 ± 2.56 µg/mL. Antiproliferative activity was observed against human tumor cell lines, whose IC50 values were 56.17, 66.43 and -65.83 µg/mL for MCF-7, HeLa and M059J cells, respectively. Its major constituents, which were determined by GC-FID and GC-MS, were carvacrol (20.7 %), acetophenone (13.5 %), spathulenol (11.0 %), (E)-nerolidol (9.7 %) and β-caryophyllene (6.2 %). These results showed the effectiveness of BGP-EO as a natural product which has promising biological activities.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Propolis
/
Anti-Infective Agents
/
Antioxidants
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2020
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal Institute of Education, Science and Technology of the South of Minas Gerais/BR
/
Federal Institute of Education, Science and Technology of the Triângulo Mineiro/BR
/
Federal University of Uberlândia/BR
/
Goiano Federal Institute/BR
/
University of Franca/BR
/
University of São Paulo/BR
Similar
MEDLINE
...
LILACS
LIS