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Biopolymer Xanthan: A New Adjuvant for DNA Vaccines
Silveira, Marcelle Moura; Conceição, Fabricio Rochedo; Mendonça, Marcelo; Moreira, Gustavo Marçal Schmidt Garcia; Cunha, Carlos Eduardo Pouey da; Rizzi, Caroline; Hartwig, Daiane Drawanz; Moreira, Angelita da Silveira; Vendrusculo, Claire Tondo; Moreira, Ângela Nunes.
  • Silveira, Marcelle Moura; Federal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas. BR
  • Conceição, Fabricio Rochedo; Federal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas. BR
  • Mendonça, Marcelo; Federal Rural University of Pernambuco. Academic Unit of Garanhuns. Garanhuns. BR
  • Moreira, Gustavo Marçal Schmidt Garcia; Federal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas. BR
  • Cunha, Carlos Eduardo Pouey da; Federal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas. BR
  • Rizzi, Caroline; Federal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas. BR
  • Hartwig, Daiane Drawanz; Federal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas. BR
  • Moreira, Angelita da Silveira; Federal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas. BR
  • Vendrusculo, Claire Tondo; Federal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas. BR
  • Moreira, Ângela Nunes; Federal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas. BR
Braz. arch. biol. technol ; 63: e20190090, 2020. graf
Article in English | LILACS | ID: biblio-1132173
ABSTRACT
Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan.
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Full text: Available Index: LILACS (Americas) Main subject: Polysaccharides, Bacterial / DNA, Recombinant / Adjuvants, Immunologic / Xanthomonas campestris / Vaccines, DNA Limits: Animals Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal Rural University of Pernambuco/BR / Federal University of Pelotas/BR

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Full text: Available Index: LILACS (Americas) Main subject: Polysaccharides, Bacterial / DNA, Recombinant / Adjuvants, Immunologic / Xanthomonas campestris / Vaccines, DNA Limits: Animals Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal Rural University of Pernambuco/BR / Federal University of Pelotas/BR