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Investigation of Protective Effect of Matricaria Chamomilla L. Extract on Methotrexate-Induced Hepatotoxicity in Wistar Rat
Afarani, Maral Soltani; Mohammadi, Mohammad; Shokri, Maryam Monsef; Mohammadzadeh, Sara.
  • Afarani, Maral Soltani; Islamic Azad University. Department of Biology. Science and Research Branch. Tehran. IR
  • Mohammadi, Mohammad; Shahid Chamran University of Ahvaz. Faculty of Science. Department of Biology. Ahvaz. IR
  • Shokri, Maryam Monsef; International Sturgeon Research Institute. Agricultural Research, Education and Extension Organization. Rasht. IR
  • Mohammadzadeh, Sara; Kermanshah University of Medical Sciences. Medical Biology Research Center. Kermanshah. IR
Braz. arch. biol. technol ; 63: e20180626, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132199
ABSTRACT
Abstract Methotrexate (MTX) was shown to cause oxidative stress and liver damage. The objective was to investigate the possible protective effects of Matricaria Chamomilla L. (chamomile) extract with anti-oxidant and anti-inflammatory properties on the methotrexate-induced liver toxicity. Twenty four Wistar rats were divided into four groups. MTX group was injected intraperitoneally on days 7 and 14 with 20 mg/kg methotrexate. Groups CE200 (chamomile extract 200 mg/kg/day) and CE300 (chamomile extract 300 mg/kg/day) received the same dose of methotrexate added with chamomile extract orally for 15 days at 200 mg/kg and 300 mg/kg respectively and the last group was healthy control group. Results of biochemical analyses indicated serum liver biomarkers (aminotransferases), alkaline phosphatase (ALP), albumin, and liver content of anti-oxidant enzymes (catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px)), reduced glutathione (GSH) and total anti-oxidant capacity (TAC) significantly increased (P <0.05-0.001) to normal in the CE treated groups compared to those of the MTX group. Serum bilirubin and hepatic malondialdehyde (MDA) levels significantly increased (P ˂0.001) in MTX group compared to those of the control group and decreased in CE200 and CE300 groups compared to those of the MTX group. Histopathological study showed inflammatory damage, necrotic cells and lipid infiltration in MTX group. In the groups treated with the chamomile extract, a significant improvement was observed in liver tissue in response to increased dose of the extract. In conclusion, chamomile extract administration could have a protective role in methotrexate-induced liver toxicity in rats through improving anti-oxidant defense system.
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Full text: Available Index: LILACS (Americas) Main subject: Plant Extracts / Methotrexate / Protective Agents / Matricaria / Liver Limits: Animals Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Iran Institution/Affiliation country: International Sturgeon Research Institute/IR / Islamic Azad University/IR / Kermanshah University of Medical Sciences/IR / Shahid Chamran University of Ahvaz/IR

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Full text: Available Index: LILACS (Americas) Main subject: Plant Extracts / Methotrexate / Protective Agents / Matricaria / Liver Limits: Animals Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Iran Institution/Affiliation country: International Sturgeon Research Institute/IR / Islamic Azad University/IR / Kermanshah University of Medical Sciences/IR / Shahid Chamran University of Ahvaz/IR