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Antifungal and Antioxidant Activities of Vernonia Chalybaea Mart. ex DC. Essential Oil and their Major Constituent β -caryophyllene
Nogueira Sobrinho, Antonio Carlos; Morais, Selene Maia de; Souza, Elnatan Bezerra de; Albuquerque, Maria Rose Jane Ribeiro; Santos, Hélcio Silva dos; Cavalcante, Carolina Sidrim de Paula; Sousa, Halisson Araújo de; Fontenelle, Raquel Oliveira dos Santos.
  • Nogueira Sobrinho, Antonio Carlos; Ceara State University. Faculty of Veterinary Medicine. Postgraduate Program in Biotechnology-Renorbio. BR
  • Morais, Selene Maia de; Ceara State University. Faculty of Veterinary Medicine. Postgraduate Program in Biotechnology-Renorbio. BR
  • Souza, Elnatan Bezerra de; Vale do Acaraú State University. Center for Agrarian and Biological Sciences. Biological Sciences Course. Sobral. BR
  • Albuquerque, Maria Rose Jane Ribeiro; Vale do Acaraú State University. Center for Exact Sciences and Technology. Chemistry Course. Sobral. BR
  • Santos, Hélcio Silva dos; Vale do Acaraú State University. Center for Exact Sciences and Technology. Chemistry Course. Sobral. BR
  • Cavalcante, Carolina Sidrim de Paula; Federal University of Ceara. Faculty of Pharmacy, Dentistry and Nursing. BR
  • Sousa, Halisson Araújo de; Ceara State University. Science and Technology Center. Chemistry Course. BR
  • Fontenelle, Raquel Oliveira dos Santos; Vale do Acaraú State University. Center for Agrarian and Biological Sciences. Biological Sciences Course. Sobral. BR
Braz. arch. biol. technol ; 63: e20190177, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132206
Responsible library: BR1.1
ABSTRACT
Abstract This article describes the chemical composition of Vernonia chalybaea essential oil, and investigates its antimicrobial, antioxidant and hemolytic activities. The evaluation of the antifungal activity was performed by the broth microdilution method using strains of yeasts and dermatophytic fungi. The checkerboard technique to find antimicrobial modulatory effects was performed using ketoconazole as standard drug. The antioxidant activity was evaluated by DPPH scavenging assay and β-carotene/linoleic-acid system. The toxicity was characterized by the brine shrimp lethality test and hemolysis bioassays. The essential oil was obtained by hydrodistillation and analyzed by GC-MS method, showing to be rich in the sesquiterpenes β-caryophyllene (39.06%) and bicyclogermacrene (19.69%), and also demonstrated a relevant antifungal activity against strains of Trichophyton rubrum. In the modulatory activity assay, the essential oil of V. chalybaea and β-caryophyllene demonstrated a synergistic interaction with ketoconazole, with increasing of its antifungal action. The antioxidant activity was evidenced mainly by β-carotene/linoleic acid system, with IC50 value of 35.87 ± 0.32 µg/mL. The results suggest that V. chalybaea essential oil and β-caryophyllene are valuable natural medicinal agents with antioxidant and antimicrobial activities.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Oils, Volatile / Vernonia / Ketoconazole / Antifungal Agents Limits: Animals / Humans Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Ceara State University/BR / Federal University of Ceara/BR / Vale do Acaraú State University/BR

Full text: Available Index: LILACS (Americas) Main subject: Oils, Volatile / Vernonia / Ketoconazole / Antifungal Agents Limits: Animals / Humans Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Ceara State University/BR / Federal University of Ceara/BR / Vale do Acaraú State University/BR