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The α -Tomatine Exhibits Antiproliferative Activity, Rupture of Cell Membranes and Induces the Expression of APC Gene in the Human Colorectal Adenocarcinoma Cell Line (Ht-29)
Ishii, Priscila Lumi; Oliveira, Rodrigo Juliano; Mauro, Mariana de Oliveira; Zorgetto-Pinheiro, Verônica Assalin; Sartori, Daniele; Lepri, Sandra Regina; Baranoski, Adrivanio; Mantovani, Mário Sérgio; Ribeiro, Lúcia Regina.
  • Ishii, Priscila Lumi; Paulista State University. Rio Claro. BR
  • Oliveira, Rodrigo Juliano; Federal University of Mato Grosso do Sul. Faculty of Medicine. Campo Grande. BR
  • Mauro, Mariana de Oliveira; Paulista State University. Rio Claro. BR
  • Zorgetto-Pinheiro, Verônica Assalin; Federal University of Mato Grosso do Sul. Faculty of Medicine. Campo Grande. BR
  • Sartori, Daniele; State University of Londrina. Londrina. BR
  • Lepri, Sandra Regina; State University of Londrina. Londrina. BR
  • Baranoski, Adrivanio; Federal University of Mato Grosso do Sul. Faculty of Medicine. Campo Grande. BR
  • Mantovani, Mário Sérgio; State University of Londrina. Londrina. BR
  • Ribeiro, Lúcia Regina; Paulista State University. Rio Claro. BR
Braz. arch. biol. technol ; 63: e20190395, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132237
ABSTRACT
Abstract The α-tomatine is a steroidal glycoalkaloid found in immature tomatoes (Lycopersicon esculentum) that has important biological functions including the inhibition of cancer cell growth and preventing metastasis. This study aimed to evaluate the effects of α-tomatine on cytotoxicity, cellular proliferation, apoptosis, and mRNA expression of APC, CCNA2, β-catenin, CASP9, BAK, BAX and BCL-XL in colorectal adenocarcinoma cell line HT-29. HT29 cells were treated with three concentrations of α-tomatine (0.1, 1 and 10 µg/mL), although only the 1 µg/mL concentration of α-tomatine was used to evaluate genetic expression patterns by real time-PCR. Results showed that α-tomatine was cytotoxic only at the 10 µg/mL concentration. Cell proliferation was significantly inhibited after the first 24 hours of treatment only with concentrations of 10 µg/mL. In contrast, there were no significant differences in apoptosis for any treatment. In the gene expression studies, only APC expression was significantly altered by α-tomatine treatment. In conclusion, α-tomatine has antiproliferative activity in the first 24h of treatment, does not induce apoptosis in this cell line and causes disruption of cell membranes, thereby increasing the expression of APC gene related to cell cycle.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Tomatine / Apoptosis / Cell Proliferation Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Mato Grosso do Sul/BR / Paulista State University/BR / State University of Londrina/BR

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Full text: Available Index: LILACS (Americas) Main subject: Tomatine / Apoptosis / Cell Proliferation Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Mato Grosso do Sul/BR / Paulista State University/BR / State University of Londrina/BR