Immunohistochemical Analysis of P-gp, LC3-II, and Cathepsin-D Associated with Histological Changes in Fish Liver: from the Impacted Environment to Clean Water
Braz. arch. biol. technol
;
63: e20190701, 2020. tab, graf
Article
in English
| LILACS
| ID: biblio-1132253
ABSTRACT
Abstract Herein we evaluated the histopathological alterations and expression patterns of multixenobiotic resistence (MXR) and autophagic proteins in liver samples of fish chronically exposed to anthropogenic contaminants in a highly polluted river, and then again after they had been transferred to good quality water. Two groups were established euthanized on the day of capture (0 h), and maintained for 30 days in a tank (30 d). The fish of 0 h presented liver with vacuolated and hypertrophic hepatocytes. Also, it was observed strong immunostaining of cathepsin-D, LC3-II and P-gp. Necrosis and apoptosis were also observed throughout the liver. Conversely, the second group (30 d) showed recovery of the liver normal histology and weak immunoreaction of the studied proteins. So, our results indicated that there was a hepatic recovery in the fish kept in good quality water, as showed by the decreased expression of cathepsin-D, LC3-II, and the MXR (P-gp). Therefore, the alterations here observed could be proposed as potential biomarkers to be tested for following the impacts of remediation or mitigation measures to environmental impacts.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cathepsin D
/
Hepatocytes
/
Fishes
/
Liver
Type of study:
Risk factors
Limits:
Animals
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2020
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of São João Del Rei/BR
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