Newly Isolated Penicillium sp. for Cellulolytic Enzyme Production in Soybean Hull Residue
Braz. arch. biol. technol
;
63: e20170710, 2020. tab, graf
Article
in English
| LILACS
| ID: biblio-1132254
ABSTRACT
Abstract (1) Background:
The aim of this study was to evaluate the production and partial characterization of xylanase and avicelase by a newly isolated Penicillium sp. in solid-state fermentation, using soybean hulls as substrate. (2)Methods:
Temperature, time, number of spores, and substrate moisture on xylanase and avicelase bioproduction were evaluated, maximizing activity with 30°C, 1x106 spores/g substrate, 14 and 7 days of fermentation with 70 and 76% substrate moisture contents, for xylanase and avicelase, respectively. (3)Results:
Different solvents, temperatures, and agitation in the enzymatic extraction were evaluated, obtaining higher activities, 430.77 and 26.77 U/g for xylanase and avicelase using 30 min extraction and 0.05 M citrate buffer solution (pH 4.5 ), respectively at 60°C and 175 rpm and 50°C and 125 rpm. The optimum pH and temperature for enzymatic activity determination were 5.3 and 50°C. Enzyme extract stability was evaluated, obtaining higher stability with pH between 4.5 and 5.5, higher temperature of up to 40°C. The kinetic thermal denaturation (Kd), half-life time, D-value, and Z-value were similar for both enzymes. The xylanase Ed value (89.1 kJ/mol) was slightly lower than the avicelase one (96.7 kJ/mol), indicating higher thermostability for avicelase. (4)Conclusion:
In this way, the production of cellulases using alternative substrates is a way to reduce production costs, since they represent about 10% of the world demand of enzymes, with application in animal feed processing, food production and breweries, textile processing, detergent and laundry production, pulp manufacturing and the production of biofuels.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Penicillium
/
Soybeans
/
Xylosidases
/
Cellulases
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2020
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Regional Integrated University of Alto Uruguay and the Missions/BR
Similar
MEDLINE
...
LILACS
LIS