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Osteoporosis affects functional activity and gene expression of osteoblastic cells derived from rat alveolar bone
Bone Research Lab, Department of Basic and Oral BiologyVargas-Sanchez, Paula Katherine; Bone Research Lab, Department of Basic and Oral BiologyFernandes, Roger Rodrigo; Furlaneto, Flávia Aparecida Chaves; Bone Research Lab, Department of Basic and Oral BiologySousa, Luiz Gustavo de; Bone Research Lab, Department of Basic and Oral BiologySiéssere, Selma; Bone Research Lab, Department of Basic and Oral BiologyBombonato-Prado, Karina Fittipaldi.
  • Bone Research Lab, Department of Basic and Oral BiologyVargas-Sanchez, Paula Katherine; Universidade de São Paulo. School of Dentistry. Bone Research Lab, Department of Basic and Oral BiologyVargas-Sanchez, Paula Katherine. Ribeirão Preto. BR
  • Bone Research Lab, Department of Basic and Oral BiologyFernandes, Roger Rodrigo; Universidade de São Paulo. School of Dentistry. Bone Research Lab, Department of Basic and Oral BiologyFernandes, Roger Rodrigo. Ribeirão Preto. BR
  • Furlaneto, Flávia Aparecida Chaves; Universidade de São Paulo. Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry. Ribeirão Preto. BR
  • Bone Research Lab, Department of Basic and Oral BiologySousa, Luiz Gustavo de; Universidade de São Paulo. School of Dentistry. Bone Research Lab, Department of Basic and Oral BiologySousa, Luiz Gustavo de. Ribeirão Preto. BR
  • Bone Research Lab, Department of Basic and Oral BiologySiéssere, Selma; Universidade de São Paulo. School of Dentistry. Bone Research Lab, Department of Basic and Oral BiologySiéssere, Selma. Ribeirão Preto. BR
  • Bone Research Lab, Department of Basic and Oral BiologyBombonato-Prado, Karina Fittipaldi; Universidade de São Paulo. School of Dentistry. Bone Research Lab, Department of Basic and Oral BiologyBombonato-Prado, Karina Fittipaldi. Ribeirão Preto. BR
Braz. dent. j ; 31(6): 617-622, Nov.-Dec. 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1132349
ABSTRACT
Abstract Recent studies suggest that osteoporosis, in addition to the damage caused in long bones, may cause deterioration in the jaws, especially in alveolar bone sites, with effects in the progress of periodontal disease as well as in bone healing. The aim of this study was to evaluate the effect of osteoporosis in the metabolism of rat alveolar bone osteoblasts. There were used 10 female rats divided in two experimental groups (Sham and OVX), which were ovariectomized and after 8 weeks euthanized to collect mandibular bone samples in order to isolate osteoblastic cells. The cells were cultured in 24-well plates to perform the in vitro experiments. After 7, 10 and 14 days, there were evaluated cell proliferation by MTT assay, in situ detection of alkaline phosphatase (ALP) as well as mineralized nodules and expression of genes associated to bone remodeling. Results showed that at 7, 10 and 14 days cell proliferation was lower for OVX group. In situ detection of ALP was higher at 7 days and lower at 10 and 14 days in OVX group. At 17 and 21 days, OVX group had a significative decrease of mineralization nodules. There was a downregulation in the expression of Alp, Bglap and Runx2 genes and an upregulation of Opg in OVX group, whereas Opn and Rankl modulation was similar between the evaluated groups. Our results suggest that osteoporosis has a deleterious effect on alveolar bone cells from ovariectomized rats, which might affect the treatment of diseases associated to the jaw bones.
RESUMO
Resumo Estudos recentes sugerem que a osteoporose, além dos danos provocados em ossos longos, pode causar deterioração dos ossos maxilares, especialmente na região do osso alveolar, com efeitos na progressão da doença periodontal assim como no reparo ósseo. O objetivo deste estudo foi avaliar o efeito da osteoporose no metabolismo de osteoblastos do osso alveolar mandibular de ratos. Foram utilizadas 10 ratas fêmeas divididas em dois grupos experimentais (Sham e OVX), que foram ovariectomizadas e após 8 semanas, eutanasiadas para coletar amostras do osso mandibular e isolar as células osteoblásticas. As células foram cultivadas em placas de cultura de 24 poços para serem realizados os experimentos in vitro. Após 7, 10 e 14 dias foram avaliados a proliferação celular pelo ensaio de MTT, detecção in situ de fosfatase alcalina (ALP) assim como de nódulos mineralizados e expressão quantitativa de genes associados à remodelação óssea. Os resultados mostraram que aos 7, 10 e 14 dias a proliferação celular foi menor para o grupo OVX. A detecção in situ de ALP foi maior aos 7 dias e menor aos 10 e 14 dias no grupo OVX. Aos 17 e 21 dias o grupo OVX apresentou uma diminuição dos nódulos mineralizados. Houve uma repressão na expressão dos genes Alp, Bglap e Runx2 e uma indução do gene Opg no grupo OVX, enquanto que a modulação dos genes Opn e Rankl foi similar entre os grupos experimentais. Nossos resultados sugerem que a osteoporose tem um efeito deletério no metabolismo de células do osso alveolar em ratas ovariectomizadas, o que pode afetar o tratamento de doenças associadas aos ossos maxilares
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Osteoporosis Limits: Animals / Female / Humans Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Osteoporosis Limits: Animals / Female / Humans Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR