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Evaluation of chemopreventive and chemotherapeutic effect of Artemisia vulgaris extract against diethylnitrosamine induced hepatocellular carcinogenesis in Balb C mice / Avaliação dos efeitos quimiopreventivo e quimioterápico do extrato de Artemisia vulgaris contra carcinogênese hepatocelular induzida por dietilnitrosamina em camundongos Balb C
Ali, S; Ejaz, M; Dar, K K; Nasreen, S; Ashraf, N; Gillani, S F; Shafi, N; Safeer, S; Khan, M A; Andleeb, S; Akhtar, N; Mughal, T A.
  • Ali, S; Government College University. Department of Zoology. Lahore. PK
  • Ejaz, M; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. PK
  • Dar, K K; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. PK
  • Nasreen, S; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. PK
  • Ashraf, N; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. PK
  • Gillani, S F; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. PK
  • Shafi, N; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. PK
  • Safeer, S; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. PK
  • Khan, M A; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. PK
  • Andleeb, S; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. PK
  • Akhtar, N; University of Azad Jammu and Kashmir. Department of Zoology. Microbial Biotechnology and Medical Toxicology Laboratory. Muzaffarabad. PK
  • Mughal, T A; Government College University. Department of Zoology. Lahore. PK
Braz. j. biol ; 80(3): 484-496, July-Sept. 2020. graf
Article in English | LILACS | ID: biblio-1132402
ABSTRACT
Abstract The main objective of current study was to investigate the chemopreventive and chemotherapeutic activity of Artemisia vulgaris extract on diethylnitrosoamine induced hepatocarcinogenesis in Balb C mice. Diethylnitrosoamine (DEN 0.9%) was prepared to induce hepatocarcinoma in Balb C mice. The extract Artemisia vulgaris (AV) was prepared by maceration technique. Mice were classified into four groups as follows Group 1 a control group (N=7) received saline solution (3.5 μl/mg), group 2 (N=14) received diethylnitrosoamine (3.5 μl/mg) intraperitoneally once in a week for eight consecutive weeks, group 3 (N=7) received only plant extract (AV 150 mg/kg (Body weight) once in a week, while group 4 (N=7) was given in combination of diethylnitrosoamine (3.5 μl/mg) and plant extract (AV 150 mg/kg (body weight). After eight weeks of DEN administration, mice of group 2 were divided into two subgroups containing seven mice each; subgroup 1 was sacrificed while subgroup 2 was treated with plant extract only (150 mg/kg (body weight)) once in a week for eight consecutive weeks. The DEN injected mice significant decline in levels of albumin with concomitant significant elevations such as aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, alpha feto protein, gamma glutamyl transferase, 5 nucleotidase, glucose-6-phosphate dehydrogenase and bilirubin. The administration of A. vulgaris significantly decreased the DEN induced hepatotoxicity. Present study revealed the potential anti-cancerous nature of Artemisia vulgaris, both in case of chemopreventive and post-treatment of A. vulgaris. Further studies are needed to explore the mechanism of prevention and therapy.
RESUMO
Resumo O objetivo principal do presente estudo foi investigar as atividades quimiopreventiva e quimioterápica do extrato de Artemisia vulgaris em hepatocarcinogênese induzida por dietilnitrosoamina (DEN) em camundongos Balb C. Dietilnitrosoamina (DEN 0,9%) foi preparada para induzir hepatocarcinoma em camundongos da linhagem Balb C. O extrato de A. vulgaris (AV) foi preparado pela técnica de maceração. Os camundongos foram classificados em quatro grupos conforme os seguintes grupo 1, grupo controle (N=7) recebeu solução salina (3,5 µl/mg); grupo 2 (N=14) recebeu dietilnitrosoamina (3,5 µl/mg) por via intraperitoneal uma vez por semana durante oito semanas consecutivas; grupo 3 (N=7) recebeu apenas o extrato vegetal (AV 150 mg/kg (peso corporal) uma vez por semana; enquanto no grupo 4 (N=7) foi administrado uma combinação de dietilnitrosoamina (3,5 μl/mg) com extrato vegetal (AV 150 mg/kg (peso corporal). Após oito semanas de administração de DEN, os camundongos do grupo 2 foram divididos em dois subgrupos, contendo sete camundongos cada um; no subgrupo 1, os animais foram sacrificados, enquanto no subgrupo 2, os animais foram tratados apenas com extrato vegetal (150 mg/kg (peso corporal)) uma vez por semana durante oito semanas consecutivas. Os camundongos nos quais foram injetados DEN apresentaram declínio significativo nos níveis de albumina, mas elevações significativas concomitantes de aspartato aminotransferase, alanina aminotransferase, lactato desidrogenase, alfa-fetoproteína, gama-glutamiltransferase, 5' nucleotidase, glicose-6-fosfato desidrogenase e bilirrubina. A administração de A. vulgaris diminuiu significativamente a hepatotoxicidade induzida pelo DEN. O presente estudo apresentou a potencialidade anticancerosa da A. vulgaris, tanto nos casos de quimioprevenção quanto no pós-tratamento da A. vulgaris. Mais estudos são necessários para explorar o mecanismo de prevenção e a terapia.
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Full text: Available Index: LILACS (Americas) Main subject: Carcinoma, Hepatocellular / Artemisia / Liver Neoplasms Limits: Animals Language: English Journal: Braz. j. biol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Pakistan Institution/Affiliation country: Government College University/PK / University of Azad Jammu and Kashmir/PK

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Full text: Available Index: LILACS (Americas) Main subject: Carcinoma, Hepatocellular / Artemisia / Liver Neoplasms Limits: Animals Language: English Journal: Braz. j. biol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Pakistan Institution/Affiliation country: Government College University/PK / University of Azad Jammu and Kashmir/PK