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Antimicrobial activity and toxicity of glass ionomer cement containing an essential oil
Nunes, J M F F; Farias, I A P; Vieira, C A; Ribeiro, T M; Sampaio, F C; Menezes, V A.
  • Nunes, J M F F; Universidade Federal da Paraíba. Centro de Ciências da Saúde. Laboratório de Biologia Bucal. João Pessoa. BR
  • Farias, I A P; Universidade Federal da Paraíba. Centro de Ciências da Saúde. Laboratório de Biologia Bucal. João Pessoa. BR
  • Vieira, C A; Universidade Federal da Paraíba. Centro de Ciências da Saúde. Laboratório de Biologia Bucal. João Pessoa. BR
  • Ribeiro, T M; Universidade Federal da Paraíba. Centro de Ciências da Saúde. Laboratório de Biologia Bucal. João Pessoa. BR
  • Sampaio, F C; Universidade Federal da Paraíba. Centro de Ciências da Saúde. Laboratório de Biologia Bucal. João Pessoa. BR
  • Menezes, V A; Universidade de Pernambuco. Faculdade de Odontologia. Departamento de Odontologia. Camaragibe. BR
Braz. j. med. biol. res ; 53(12): e9468, 2020. graf
Article in English | LILACS, ColecionaSUS | ID: biblio-1132501
ABSTRACT
The aim of this study was to evaluate the antimicrobial activity and toxicity of glass ionomer cement (GIC) modified with 5-methyl-2-(1-methylethyl)phenol (thymol) against Streptococcus mutans in silico and in vitro. The antimicrobial activity of thymol on GIC modified with concentrations of 2% (GIC-2) and 4% (GIC-4) was evaluated in a model of planktonic cell biofilm using agar diffusion test, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), dynamic biofilm (continuous flow cell parallel), and bacterial kinetics. Conventional GIC (GIC-0) was used as a control. Thymol toxicity was evaluated in Artemia salina and in silico using Osiris® software. Differences between groups were estimated by analysis of variance, followed by Tukey post hoc test, with a 5% significance level. The results of the agar diffusion test between groups were not significantly different (P≥0.05). Thymol had potential bacteriostatic and bactericidal activity against Streptococcus mutans with respect to planktonic growth, with MIC of 100 µg/mL and MBC of 400 µg/mL. The groups GIC-0, GIC-2, and GIC-4 reduced the biofilm by approximately 10, 85, and 95%, respectively. Bacterial kinetics showed efficiency of the modified GICs for up to 96 h. GIC with thymol was effective against S. mutans, with significant inhibition of the biofilms. Analyses in silico and using Artemia salina resulted in no relevant toxicity, suggesting potential for use in humans. GIC-2 was effective against S. mutans biofilm, with decreased cell viability.
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Full text: Available Index: LILACS (Americas) Main subject: Anti-Infective Agents Limits: Humans Language: English Journal: Braz. j. med. biol. res Year: 2020 Type: Article Institution/Affiliation country: Universidade Federal da Paraíba/BR / Universidade de Pernambuco/BR

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Full text: Available Index: LILACS (Americas) Main subject: Anti-Infective Agents Limits: Humans Language: English Journal: Braz. j. med. biol. res Year: 2020 Type: Article Institution/Affiliation country: Universidade Federal da Paraíba/BR / Universidade de Pernambuco/BR