Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans
Braz. oral res. (Online)
;
34: e050, 2020. graf
Article
in English
| LILACS, BBO
| ID: biblio-1132693
ABSTRACT
Abstract Candida infection is an important cause of morbidity and mortality in immunocompromised patients. The increase in its incidence has been associated with resistance to antimicrobial therapy and biofilm formation. The aim of this study was to evaluate the efficacy of tea tree oil (TTO) and its main component - terpinen-4-ol - against resistant Candida albicans strains (genotypes A and B) identified by molecular typing and against C. albicans ATCC 90028 and SC 5314 reference strains in planktonic and biofilm cultures. The minimum inhibitory concentration, minimum fungicidal concentration, and rate of biofilm development were used to evaluate antifungal activity. Results were obtained from analysis of the biofilm using the cell proliferation assay 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and confocal laser scanning microscopy (CLSM). Terpinen-4-ol and TTO inhibited C. albicans growth. CLSM confirmed that 17.92 mg/mL of TTO and 8.86 mg/mL of terpinen-4-ol applied for 60 s (rinse simulation) interfered with biofilm formation. Hence, this in vitro study revealed that natural substances such as TTO and terpinen-4-ol present promising results for the treatment of oral candidiasis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Terpenes
/
Candida albicans
/
Biofilms
/
Tea Tree Oil
Type of study:
Evaluation studies
/
Prognostic study
Language:
English
Journal:
Braz. oral res. (Online)
Journal subject:
Dentistry
Year:
2020
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual Paulista/BR
/
Universidade Estadual de Campinas/BR
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