Inhibition of lipopolysaccharide-induced inflammation via the protective role of T regulatory cells in the fetal liver in a late-pregnancy preterm mouse model
Clinics
;
75: e1665, 2020. tab, graf
Article
in English
| LILACS
| ID: biblio-1133413
ABSTRACT
OBJECTIVES:
This study intended to explore the effect of T regulatory cells (Tregs) in the perinatal liver against LPS-induced inflammation in a preterm birth mouse model. Moreover, the role of adoptive Tregs on the inflammatory response induced by LPS was also studied.METHODS:
Female BALB/C mice were injected intraperitoneally (IP) with LPS dissolved in normal saline solution at a dose of 50 µg/kg. Spleens from pregnant mice were used to obtain Tregs. The expression of Forkhead family transcription factor-3 (Foxp3), Interleukin-6 (IL-6), Toll-like receptor-4 (TLR-4), and Heme oxygenase-1 (HO-1) were assessed from fetal liver tissues by polymerase chain reaction and western blotting.RESULTS:
LPS administered to mice induced an inflammatory response in the perinatal liver, and this inflammatory response was negatively regulated by Tregs in the experimental group. Maternal-fetal tolerance was maintained by Tregs. Transmission of Tregs was estimated in different experimental groups based on the mRNA expression of TLR-4, IL-6, HO-1, and Foxp3.CONCLUSIONS:
After analysis of the experimental data, it was determined that Tregs exhibited regulatory potential against LPS-induced inflammatory response. Further, it was concluded that the transmission of Tregs improved the mother's immune tolerance against LPS-induced inflammation in the fetal liver.
Full text:
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Index:
LILACS (Americas)
Main subject:
Lipopolysaccharides
/
Premature Birth
Type of study:
Prognostic study
Limits:
Animals
/
Pregnancy
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2020
Type:
Article
Affiliation country:
China
/
Pakistan
/
United States
Institution/Affiliation country:
Akhtar Saeed Trust Hospital & Farooq Hospital Lahore/PK
/
Boston Childrens Hospital of Harvard Medical School/US
/
Center Xian Jiaotong University Health Science Centre/CN
/
The First Affiliated Hospital of Xian Jiaotong University College of Medicine/CN
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