Your browser doesn't support javascript.
loading
Ketamine ameliorates hypoxia-induced endothelial injury in human umbilical vein endothelial cells
Zhou, Xiaohui; Liu, Jing; Yang, Siyi; Su, Yanguang; Meng, Zhipeng; Hu, Yuqin.
  • Zhou, Xiaohui; Affiliated Central Hospital of HuZhou University, 198 Hongqi Road. Department of Endocrinology, Huzhou Central Hospital. Huzhou 31300. CN
  • Liu, Jing; Huzhou Maternal & Child Health Care Hospital. Department of Anesthesia. Huzhou 313000. CN
  • Yang, Siyi; HuZhou University, Emergency Department of Huzhou Central Hospital, 198 Hongqi Road. Affiliated Central Hospital. Huzhou 31300. CN
  • Su, Yanguang; Affiliated Central Hospital of HuZhou University, 198 Hongqi Road. Anesthesiology Department, Huzhou Central Hospital. Huzhou 31300. CN
  • Meng, Zhipeng; Affiliated Central Hospital of HuZhou University, 198 Hongqi Road. Anesthesiology Department, Huzhou Central Hospital. Huzhou 31300. CN
  • Hu, Yuqin; Affiliated Central Hospital of HuZhou University, 198 Hongqi Road. Anesthesiology Department, Huzhou Central Hospital. Huzhou 31300. CN
Clinics ; 75: e1865, 2020. graf
Article in English | LILACS | ID: biblio-1133469
ABSTRACT

OBJECTIVES:

Hypoxia leads to endothelial cell inflammation, apoptosis, and damage, which plays an important role in the complications associated with ischemic cardiovascular disease. As an oxidoreductase, p66Shc plays an important role in the regulation of reactive oxygen species (ROS) production and apoptosis. Ketamine is widely used in clinics. This study was designed to assess the potential protective effect of ketamine against hypoxia-induced injury in human umbilical vein endothelial cells (HUVECs). Moreover, we explored the potential mechanism by which ketamine protected against hypoxia-induced endothelial injury.

METHODS:

The protective effects of ketamine against hypoxia-induced injury was assessed using cell viability and adhesion assays, quantitative polymerase chain reaction, and western blotting.

RESULTS:

Our data showed that hypoxia reduced HUVEC viability, increased the adhesion between HUVECs and monocytes, and upregulated the expression of endothelial adhesion molecules at the protein and mRNA levels. Moreover, hypoxia increased ROS accumulation and upregulated p66Shc expression. Furthermore, hypoxia downregulated sirt1 expression in HUVECs. Alternatively, ketamine was shown to reverse the hypoxia-mediated reduction of cell viability and increase in the adhesion between HUVECs and monocytes, ameliorate hypoxia-induced ROS accumulation, and suppress p66Shc expression. Moreover, EX527, a sirt1 inhibitor, reversed the protective effects of ketamine against the hypoxia-mediated reduction of cell viability and increase in adhesion between HUVECs and monocytes.

CONCLUSION:

Ketamine reduces hypoxia-induced p66Shc expression and attenuates ROS accumulation via upregulating sirt1 in HUVECs, thus attenuating hypoxia-induced endothelial cell inflammation and apoptosis.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Reactive Oxygen Species / Apoptosis / Human Umbilical Vein Endothelial Cells / Ketamine / Hypoxia Limits: Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Affiliated Central Hospital of HuZhou University, 198 Hongqi Road/CN / HuZhou University, Emergency Department of Huzhou Central Hospital, 198 Hongqi Road/CN / Huzhou Maternal & Child Health Care Hospital/CN

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Reactive Oxygen Species / Apoptosis / Human Umbilical Vein Endothelial Cells / Ketamine / Hypoxia Limits: Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Affiliated Central Hospital of HuZhou University, 198 Hongqi Road/CN / HuZhou University, Emergency Department of Huzhou Central Hospital, 198 Hongqi Road/CN / Huzhou Maternal & Child Health Care Hospital/CN