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Histological comparison of two special methods of staining melanin in human skin / Comparación histológica de dos métodos especiales de tinción de melanina en piel humana
Shataer, Munire; Shataer, Saimire; Libin, Liao; Tian, Li; Shengbin, Bai.
  • Shataer, Munire; Xinjiang Medical University. Basic Medical College. Department of Histology and Embryology. Ürümqi. CN
  • Shataer, Saimire; Xinjiang Medical University. the Second Affiliated Hospital. Department of Neurology. Ürümqi. CN
  • Libin, Liao; Xinjiang Medical University. Basic Medical College. Department of Histology and Embryology. Ürümqi. CN
  • Tian, Li; Xinjiang Medical University. Basic Medical College. Department of Histology and Embryology. Ürümqi. CN
  • Shengbin, Bai; Xinjiang Medical University. Basic Medical College. Department of Histology and Embryology. Ürümqi. CN
Int. j. morphol ; 38(6): 1535-1538, Dec. 2020. tab, graf
Article in English | LILACS | ID: biblio-1134473
ABSTRACT

SUMMARY:

Human skin melanin was stained using the Fontana's silver nitrate method and Schmorl method. The results showed that, in the Fontana's silver nitrate method, melanin and silver-bound cells were black and other tissues were red. When stained using the Schmorl method, effects on melanin differed based on whether the nuclei were stained. When the nucleus was stained, melanin appeared blue-black or blue-green, and other tissue structures were purple. When the nucleus was not stained, melanin was orange and other structures were pink. Comparing the two staining methods, we concluded that Fontana's silver nitrate method takes a long time; in contrast, the Schmorl method showed two different types of results depending on whether the nucleus was stained, and it takes less time than Fontana staining, so we here consider the Schmorl method more suitable for special staining of melanin than Fontana's silver nitrate method.
RESUMEN
RESUMEN La melanina de la piel humana se tiñó utilizando el método del nitrato de plata de Fontana y el método Schmorl. Los resultados mostraron que, en el método del nitrato de plata de Fontana, la melanina y las células unidas a plata eran negras y otros tejidos eran rojos. Cuando se tiñó con el método de Schmorl, los efectos sobre la melanina difirieron en función de si se tiñeron los núcleos. Cuando se tiñó el núcleo, la melanina apareció de color azul-negro o azul-verde, y otras estructuras de tejido fueron de color púrpura. Cuando el núcleo no estaba teñido, la melanina era naranja y otras estructuras eran rosadas. Al comparar los dos métodos de tinción, llegamos a la conclusión de que el método del nitrato de plata de Fontana lleva mucho tiempo; por el contrario, el método Schmorl mostró dos tipos diferentes de resultados dependiendo de si el núcleo estaba teñido, y lleva menos tiempo que la tinción de Fontana, por lo que aquí consideramos que el método Schmorl es más adecuado para la tinción especial de melanina que el método del nitrato de plata de Fontana.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Silver Nitrate / Skin / Staining and Labeling / Melanins Limits: Humans Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Xinjiang Medical University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Silver Nitrate / Skin / Staining and Labeling / Melanins Limits: Humans Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Xinjiang Medical University/CN