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Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 associated with apoptosis in the corpus cavernosum of rats subjected to chronic alcoholism model / Expresión de AIF, PARP y miRNAs MIR-145, MIR-210 y MIR-486 asociados con apoptosis en el cuerpo cavernoso de ratas sometidas a modelo de alcoholismo crónico
Sarraipo, Vagner Schiavoni; Lizarte Neto, Fermino Sanches; Carvalho, Camila Albuquerque Mello de; Silva, Jairo Pinheiro da; Durand, Marina Toledo; Schimming, Bruno Cezar; Martinez, Marcelo; Galli, Maria de Fátima Tazima Sorita; Buono, Fabianna de Oliveira; Tiezzi, Maria Fernanda Barbirato da Mata; Novais, Paulo Cezar; Gula, Isabella Stracieri; Thomazini, José Antônio; Fazan, Valeria Paula Sassoli; Rodrigues, Andressa Romualdo; Tirapelli, Daniela Pretti da Cunha; Cirino, Mucio Luiz de Assis; Molina, Carlos Augusto Fernandes; Junior, Silvio Tucci; Tirapelli, Luis Fernando.
  • Sarraipo, Vagner Schiavoni; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Lizarte Neto, Fermino Sanches; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Carvalho, Camila Albuquerque Mello de; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Silva, Jairo Pinheiro da; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Durand, Marina Toledo; University of Ribeirão Preto. Department of Medicine. BR
  • Schimming, Bruno Cezar; UNESP. Biosciences Institute of Botucatu. Department of Anatomy. Botucatu. BR
  • Martinez, Marcelo; UFSCAR. Department of Morphology and pathology. São Carlos. BR
  • Galli, Maria de Fátima Tazima Sorita; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Buono, Fabianna de Oliveira; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Tiezzi, Maria Fernanda Barbirato da Mata; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Novais, Paulo Cezar; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Gula, Isabella Stracieri; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Thomazini, José Antônio; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Fazan, Valeria Paula Sassoli; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Rodrigues, Andressa Romualdo; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Tirapelli, Daniela Pretti da Cunha; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Cirino, Mucio Luiz de Assis; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Molina, Carlos Augusto Fernandes; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Junior, Silvio Tucci; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
  • Tirapelli, Luis Fernando; São Paulo Univesity. Medical School of Ribeirão Preto. Department of Surgery and Anatomy. BR
Int. j. morphol ; 38(6): 1639-1644, Dec. 2020. graf
Article in English | LILACS | ID: biblio-1134491
Responsible library: CL1.1
ABSTRACT

SUMMARY:

Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs miR-145, miR-210 and miR-486, in the corpus cavernosum of rats submitted to a semivoluntary alcoholism model. For this study 24 Wistar rats were divided into two groups control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression of AIF and PARP and their regulatory microRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoluntary alcoholism.
RESUMEN
RESUMEN Estudios previos de nuestro grupo describieron las consecuencias del uso de etanol en la erección del pene. Sin embargo, los mecanismos moleculares que rodean a los microARN, el proceso de apoptosis y su relación con la disfunción eréctil asociada con el consumo de alcohol aún no se conocen bien. El objetivo de este análisis fue evaluar el mecanismo de apoptosis mediante la expresión de AIF y PARP, así como sus microARN reguladores miR-145, miR-210 y miR-486, en el cuerpo cavernoso de ratas sometidas a un modelo de alcoholismo semivoluntario. Se dividieron 24 ratas Wistar en dos grupos control (C) grupo de ratas tratadas con etanol al 20 % (A) durante siete semanas. Las muestras del cuerpo cavernoso se prepararon para el análisis inmunohistoquímico de la expresión de la proteína AIF y PARP, y la expresión del gen microRNAs miR-145, miR-210, miR-486 en tejido cavernoso se realizó por PCR en tiempo real. El análisis inmunohistoquímico mostró escaso etiquetado nuclear positivo para la proteína PARP y AIF en el cuerpo cavernoso de los animales de control y tratados con etanol. Después del análisis de la expresión de microARN miR-145, -210 y -486 no se encontraron resultados con diferencias estadísticas significativas entre los grupos control y alcoholizados. La expresión de AIF y PARP y sus microARN reguladores involucrados en el proceso apoptótico (miR-145, miR-210 y miR-486) no se alteraron en el cuerpo cavernoso de las ratas sometidas a alcoholismo semivoluntario.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Apoptosis / Alcoholism / Erectile Dysfunction Type of study: Risk factors Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: São Paulo Univesity/BR / UFSCAR/BR / UNESP/BR / University of Ribeirão Preto/BR

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Full text: Available Index: LILACS (Americas) Main subject: Apoptosis / Alcoholism / Erectile Dysfunction Type of study: Risk factors Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: São Paulo Univesity/BR / UFSCAR/BR / UNESP/BR / University of Ribeirão Preto/BR