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Mitochondrial activity disruption and local muscle damage induced in mice by Scolopendra polymorpha venom
Robles, Judith Tabullo De; Valverde, Francisca Fernández; Cisneros, Lucero Valladares; Villeda, Juana Hernández; Sánchez-Reyes, Ayixon; Gutiérrez, María del Carmen.
  • Robles, Judith Tabullo De; Autonomous University of the State of Morelos. Center of Biotechnology Research. Cuernavaca. MX
  • Valverde, Francisca Fernández; National Institute of Neurology and Neurosurgery. México City. MX
  • Cisneros, Lucero Valladares; Autonomous University of the State of Morelos. Center of Biotechnology Research. Cuernavaca. MX
  • Villeda, Juana Hernández; National Institute of Neurology and Neurosurgery. México City. MX
  • Sánchez-Reyes, Ayixon; National Autonomous University of Mexico. Cátedras Conacyt-Institute of Biotechnology. Cuernavaca. MX
  • Gutiérrez, María del Carmen; Autonomous University of the State of Morelos. Center of Biotechnology Research. Cuernavaca. MX
J. venom. anim. toxins incl. trop. dis ; 26: e20190079, 2020. graf, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1135133
ABSTRACT
Scolopendra polymorpha (S. polymorpha) is a predatory centipede whose venom contains a multiplicity of biochemical effectors that can cause muscle damage and cumulative cell destruction in its prey. Despite previous investigations of S. polymorpha and other centipede venoms, there is a lack of information on the morphological and biochemical patterns elicited by their myotoxic effects. To elucidate these processes, this paper presents evidence of skeletal muscle damage, and alterations in key biochemical mediators that appear only after exposure to centipede venom.

Methods:

Venom was collected and fractionated using RP-HPLC; mouse extensor digitorum longus (EDL) muscle was exposed to whole venom and venom fractions to evaluate myotoxicity by means of creatine kinase (CK) - a muscle damage marker - activity measurements and histochemical analysis.

Results:

CK activity was higher in EDL muscle exposed to venom than in unexposed muscle. This increase was observed after 15 min of venom incubation, and remained stable up to 45 min. Venom-exposed EDL muscle showed signs of muscle damage including necrosis, loss of fascicular structure as well as mitochondrial accumulations and ragged red fibers (RRF), suggesting an impairment in the normal mitochondrial arrangement. Nicotinamide adenine dinucleotide (NADH) and cytochrome oxidase (COX) tests also indicate that respiratory complexes might be affected.

Conclusion:

Our results suggest a different biochemical composition of S. polymorpha venom, based on the different effects of four venom fractions on the cells tested, according to statistical evidence. Fractions F6 and F7 caused the most important alterations.(AU)
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Creatine Kinase / Myotoxicity / Chilopoda Limits: Animals Language: English Journal: J. venom. anim. toxins incl. trop. dis Year: 2020 Type: Article Institution/Affiliation country: Autonomous University of the State of Morelos/MX / National Autonomous University of Mexico/MX / National Institute of Neurology and Neurosurgery/MX

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Full text: Available Index: LILACS (Americas) Main subject: Creatine Kinase / Myotoxicity / Chilopoda Limits: Animals Language: English Journal: J. venom. anim. toxins incl. trop. dis Year: 2020 Type: Article Institution/Affiliation country: Autonomous University of the State of Morelos/MX / National Autonomous University of Mexico/MX / National Institute of Neurology and Neurosurgery/MX