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Evaluation of different total Leishmania amazonensis antigens for the development of a first-generation vaccine formulated with a Toll-like receptor-3 agonist to prevent cutaneous leishmaniasis
Germanó, María José; Lozano, Esteban Sebastián; Sanchez, María Victoria; Bruna, Flavia Alejandra; García-Bustos, María Fernanda; Sosa Lochedino, Arianna Lourdes; Salomón, María Cristina; Fernandes, Ana Paula; Mackern-Oberti, Juan Pablo; Cargnelutti, Diego Esteban.
  • Germanó, María José; Consejo Nacional de Investigaciones Científicas y Tecnológicas. Universidad Nacional de Cuyo. Instituto de Medicina y Biología Experimental de Cuyo. Mendoza. AR
  • Lozano, Esteban Sebastián; Consejo Nacional de Investigaciones Científicas y Tecnológicas. Universidad Nacional de Cuyo. Instituto de Medicina y Biología Experimental de Cuyo. Mendoza. AR
  • Sanchez, María Victoria; Consejo Nacional de Investigaciones Científicas y Tecnológicas. Universidad Nacional de Cuyo. Instituto de Medicina y Biología Experimental de Cuyo. Mendoza. AR
  • Bruna, Flavia Alejandra; Consejo Nacional de Investigaciones Científicas y Tecnológicas. Universidad Nacional de Cuyo. Instituto de Medicina y Biología Experimental de Cuyo. Mendoza. AR
  • García-Bustos, María Fernanda; Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Patología Experimental. Salta. AR
  • Sosa Lochedino, Arianna Lourdes; Consejo Nacional de Investigaciones Científicas y Tecnológicas. Universidad Nacional de Cuyo. Instituto de Medicina y Biología Experimental de Cuyo. Mendoza. AR
  • Salomón, María Cristina; Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Mendoza. AR
  • Fernandes, Ana Paula; Universidade Federal de Minas Gerais. Faculdade de Farmácia. Belo Horizonte. BR
  • Mackern-Oberti, Juan Pablo; Consejo Nacional de Investigaciones Científicas y Tecnológicas. Universidad Nacional de Cuyo. Instituto de Medicina y Biología Experimental de Cuyo. Mendoza. AR
  • Cargnelutti, Diego Esteban; Consejo Nacional de Investigaciones Científicas y Tecnológicas. Universidad Nacional de Cuyo. Instituto de Medicina y Biología Experimental de Cuyo. Mendoza. AR
Mem. Inst. Oswaldo Cruz ; 115: e200067, 2020. graf
Article in English | LILACS, SES-SP | ID: biblio-1135224
Responsible library: BR1.1
ABSTRACT
BACKGROUND Unfortunately, no any vaccine against leishmaniasis has been developed for human use. Therefore, a vaccine based on total Leishmania antigens could be a good and economic approach; and there are different methodologies to obtain these antigens. However, it is unknown whether the method to obtain the antigens affects the integrity and immune response caused by them. OBJECTIVES to compare the protein profile and immune response generated by total L. amazonensis antigens (TLA) produced by different methods, as well as to analyse the immune response and protection by a first-generation vaccine formulated with sonicated TLA (sTLA) and polyinosinicpolycytidylic acid [Poly (IC)]. METHODS TLA were obtained by four different methodologies and their integrity and immune response were evaluated. Finally, sTLA was formulated with Poly (IC) and their protective immune response was measured. FINDINGS sTLA presented a conserved protein profile and induced a strong immune response. In addition, Poly (IC) improved the immune response generated by sTLA. Finally, sTLA + Poly (IC) formulation provided partial protection against L. amazonensis infection. MAIN CONCLUSIONS The protein profile and immune response depend on the methodology used to obtain the antigens. Also, the formulation sTLA + Poly (IC) provides partial protection against cutaneous leishmaniasis in mice.
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Full text: Available Index: LILACS (Americas) Main subject: Protozoan Vaccines / Leishmaniasis, Cutaneous / Toll-Like Receptor 3 / Leishmaniasis Vaccines / Leishmania Limits: Animals / Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Year: 2020 Type: Article Institution/Affiliation country: Consejo Nacional de Investigaciones Científicas y Tecnológicas/AR / Universidad Nacional de Cuyo/AR / Universidade Federal de Minas Gerais/BR

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Full text: Available Index: LILACS (Americas) Main subject: Protozoan Vaccines / Leishmaniasis, Cutaneous / Toll-Like Receptor 3 / Leishmaniasis Vaccines / Leishmania Limits: Animals / Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Year: 2020 Type: Article Institution/Affiliation country: Consejo Nacional de Investigaciones Científicas y Tecnológicas/AR / Universidad Nacional de Cuyo/AR / Universidade Federal de Minas Gerais/BR