In vitro-induction of metronidazole-resistant Giardia duodenalis is not associated with nucleotide alterations in the genes involved in pro-drug activation
Mem. Inst. Oswaldo Cruz
;
115: e200303, 2020. tab, graf
Article
in English
| LILACS, SES-SP
| ID: biblio-1135270
ABSTRACT
Giardiasis is an infectious disease caused by Giardia duodenalis. The pro-drug metronidazole (MTZ) is the first-line treatment for giardiasis. Parasite's proteins as pyruvateferredoxin oxidoreductase (PFOR), ferredoxin (Fd), nitroreductase-1 (NR-1) and thioredoxin reductase (TrxR) participate in MTZ activation. Here, we showed Giardia trophozoites long-term exposed to MTZ presented higher IC50 than controls, showing the drug influenced the parasite survival. That reduction in MTZ's susceptibility does not seem to be related to mutations in the genes pfor, fd, nr-1 or trxr. It points that different mechanism as alterations in other metabolic pathways can account for Giardia resistance to MTZ therapy.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Drug Resistance
/
Prodrugs
/
Giardia lamblia
/
Metronidazole
/
Antiprotozoal Agents
Type of study:
Risk factors
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Year:
2020
Type:
Article
Institution/Affiliation country:
Fundação Oswaldo Cruz-Fiocruz/BR
Similar
MEDLINE
...
LILACS
LIS