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Computational prediction and characterisation of miRNAs and their pathway genes in human schistosomiasis caused by Schistosoma haematobium
Cardoso, Thaís Cunha de Sousa; de Araújo, Carlos Bruno; Portilho, Laysa Gomes; Mendes, Luiz Guilherme Alves; Alves, Tamires Caixeta; Silva, Gustavo Caetano; Laboratório de Fisiologia Molecular de PlantasRibeiro, Thales Henrique Cherubino; Gandolfi, Peterson Elizandro; Morais, Enyara Rezende; do Amaral, Laurence Rodrigues; Gomes, Matheus de Souza.
  • Cardoso, Thaís Cunha de Sousa; Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas. BR
  • de Araújo, Carlos Bruno; Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas. BR
  • Portilho, Laysa Gomes; Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas. BR
  • Mendes, Luiz Guilherme Alves; Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas. BR
  • Alves, Tamires Caixeta; Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas. BR
  • Silva, Gustavo Caetano; Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas. BR
  • Laboratório de Fisiologia Molecular de PlantasRibeiro, Thales Henrique Cherubino; Universidade Federal de Lavras. Departamento de Biologia, Setor de Fisiologia Vegetal. Laboratório de Fisiologia Molecular de PlantasRibeiro, Thales Henrique Cherubino. Lavras. BR
  • Gandolfi, Peterson Elizandro; Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas. BR
  • Morais, Enyara Rezende; Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas. BR
  • do Amaral, Laurence Rodrigues; Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas. BR
  • Gomes, Matheus de Souza; Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas. BR
Mem. Inst. Oswaldo Cruz ; 115: e190378, 2020. tab, graf
Article in English | LILACS, SES-SP | ID: biblio-1135284
ABSTRACT
BACKGROUND Key genes control the infectivity of the Schistosoma haematobium causing schistosomiasis. A method for understanding the regulation of these genes might help in developing new disease strategies to control schistosomiasis, such as the silencing mediated by microRNAs (miRNAs). The miRNAs have been studied in schistosome species and they play important roles in the post-transcriptional regulation of genes, and in parasite-host interactions. However, genome-wide identification and characterisation of novel miRNAs and their pathway genes and their gene expression have not been explored deeply in the genome and transcriptome of S. haematobium. OBJECTIVES Identify and characterise mature and precursor miRNAs and their pathway genes in the S. haematobium genome. METHODS Computational prediction and characterisation of miRNAs and genes involved in miRNA pathway from S. haematobium genome on SchistoDB. Conserved domain analysis was performed using PFAM and CDD databases. A robust algorithm was applied to identify mature miRNAs and their precursors. The characterisation of the precursor miRNAs was performed using RNAfold, RNAalifold and Perl scripts. FINDINGS We identified and characterised 14 putative proteins involved in miRNA pathway including ARGONAUTE and DICER in S. haematobium. Besides that, 149 mature miRNAs and 131 precursor miRNAs were identified in the genome including novel miRNAs. MAIN CONCLUSIONS miRNA pathway occurs in the S. haematobium, including endogenous miRNAs and miRNA pathway components, suggesting a role of this type of non-coding RNAs in gene regulation in the parasite. The results found in this work will open up a new avenue for studying miRNAs in the S. haematobium biology in helping to understand the mechanism of gene silencing in the human parasite Schistosome.
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Full text: Available Index: LILACS (Americas) Main subject: Schistosoma haematobium / Schistosomiasis / Gene Expression Regulation / Computational Biology / MicroRNAs Type of study: Prognostic study / Risk factors Limits: Animals / Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Year: 2020 Type: Article Institution/Affiliation country: Universidade Federal de Lavras/BR / Universidade Federal de Uberlândia/BR

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Full text: Available Index: LILACS (Americas) Main subject: Schistosoma haematobium / Schistosomiasis / Gene Expression Regulation / Computational Biology / MicroRNAs Type of study: Prognostic study / Risk factors Limits: Animals / Humans Language: English Journal: Mem. Inst. Oswaldo Cruz Year: 2020 Type: Article Institution/Affiliation country: Universidade Federal de Lavras/BR / Universidade Federal de Uberlândia/BR