Differences in Proteomic Profiles Between Caries Free and Caries Affected Children
Pesqui. bras. odontopediatria clín. integr
;
20: e5592, 2020. tab
Article
in English
| LILACS, BBO
| ID: biblio-1135503
ABSTRACT
Abstract Objective:
To determine if there are differences in protein profiles in saliva depending if children of caries-free versus caries affected. Material andMethods:
A cohort of 91 children with ages between 6 and 19 years, along clinical status of caries experience. Protein profiles in saliva were determined using electrophoresis and the calculation of the percentage of a specific band at a specific molecular weight in relationship to the total protein in that sample (% of total) using molecular weight standards. This quantification was repeated for each protein band across a range of molecular weights for each sample. Chi-square, Fisher's exact, and Student t-tests were used to compare the distributions between caries-free and caries affected children (α=0.05).Results:
Histatin was more likely to be non-detectable or reduced in caries-free children (OR=7.56; 95% CI 1.62-35.13) and these children had on average one less gel band detected by the assay we used.Conclusion:
We have found differences in proteins between caries affected and caries-free children, suggesting that this line of investigation holds the promise of providing new tools for caries management.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Saliva
/
Dental Caries
/
Proteomics
/
Electrophoresis
Type of study:
Practice guideline
Limits:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Country/Region as subject:
North America
Language:
English
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
Dentistry
Year:
2020
Type:
Article
Affiliation country:
United States
Institution/Affiliation country:
University of Arizona/US
/
University of Pittsburgh/US
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