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Nuclear factor erythroid-2 related factor 2 inhibits human disc nucleus pulpous cells apoptosis induced by excessive hydrogen peroxide
Lin, Hao; Wang, Yingxin; Jing, Kaipeng; Wu, Tingrui; Niu, Yanru; Wei, Jinsong.
  • Lin, Hao; Guangdong Medical University. Affiliated Hospital. Orthopaedic Center. Zhanjiang. CN
  • Wang, Yingxin; Guangdong Medical University. Affiliated Hospital. Orthopaedic Center. Zhanjiang. CN
  • Jing, Kaipeng; Guangdong Medical University. Affiliated Hospital. Department of Nephrology. Zhanjiang. CN
  • Wu, Tingrui; Guangdong Medical University. Affiliated Hospital. Orthopaedic Center. Zhanjiang. CN
  • Niu, Yanru; Guangdong Medical University. Affiliated Hospital. Orthopaedic Center. Zhanjiang. CN
  • Wei, Jinsong; Guangdong Medical University. Affiliated Hospital. Orthopaedic Center. Zhanjiang. CN
Rev Assoc Med Bras (1992) ; 66(7): 986-991, 2020. graf
Article in English | SES-SP, LILACS | ID: biblio-1136303
ABSTRACT
SUMMARY OBJECTIVE Nuclear factor erythroid-2 related factor 2 (Nrf2)/ antioxidant response element (ARE) is a novel defensive pathway involved in the oxidative and chemical stress of cells. The aim of the study was to explore the role of Nrf2 on the apoptosis of human disc nucleus pulpous cells induced by hydrogen peroxide (H2O2). METHODS The degeneration model of human intervertebral disc nucleus pulpous cells was established. The expression of Nrf2 was interfered with using sulforaphane (SFN); for that end, three groups were established a blank group (H2O2-/SFN-), control group (H2O2+/SFN-), and an experimental group (H2O2+/SFN+). CCK8, Hoechst 33258 living cell staining was used to detect reactive oxygen species (ROS) content. RESULTS The apoptotic rates of the three groups were [(0.40±0.46)%], [(25.98±11.28)%], and [(3.83±2.06)%, respectively. The difference was statistically significant (p<0.05). The relative content of ROS in the three groups was [(100±7)%], [(1538±91)%], and [(818±63)%]; the difference was statistically significant (p<0.05). In Western blotting, Nrf2 content in the experimental group was higher than that in the control group. CONCLUSION Nrf2 exists in the nucleus pulpous cells of human intervertebral discs, which is related to the degeneration of the intervertebral disc. It has negative feedback regulation and can prevent the degeneration of the intervertebral disc by inhibiting the apoptosis of nucleus pulpous cells of human intervertebral discs caused by excessive ROS, which provides a new intervention strategy for the prevention and treatment of the degeneration of intervertebral discs.
RESUMO
RESUMO OBJETIVO O fator 2 relacionado a NF-E2 (Nrf2)/elemento de resposta antioxidante (ARE) é uma nova via defensiva envolvida no estresse oxidativo e químico das células. O objetivo deste estudo foi explorar o papel do Nrf2 na apoptose das células do núcleo pulposo do disco humano induzida pelo peróxido de hidrogênio (H2O2). MÉTODOS O modelo de degeneração das células do núcleo pulposo do disco intervertebral humano foi estabelecido. A expressão do Nrf2 foi interferida utilizando-se sulforafano (SFN). Para isso foram estabelecidos três grupos um grupo vazio (H2O2-/SFN-), um grupo de controle (H2O2+/SFN-), e um grupo experimental (H2O2+/SFN+). Utilizando CCK8 e Hoechst 33258, o conteúdo de espécies reativas de oxigênio (ERO) foi detectado. RESULTADOS As taxas de apoptose dos três grupos foram [(0,40 ± 0,46)%], [(25,98 ± 11,28%)] e [(3,83 ± 2,06)%], respectivamente. A diferença apresentou significância estatística (p < 0,05). O conteúdo relativo de ERO nos três grupos foi [(100±7)%], [(1538±91%)], e [(818±63%); a diferença foi estatisticamente significativa (p < 0,05). O método de Western blotting indicou um maior conteúdo de Nrf2 no grupo experimental do que no grupo de controle. CONCLUSÃO O Nrf2 existe em células do núcleo pulposo do disco intervertebral humano, que estão relacionadas à degeneração do disco intervertebral. Ele apresenta regulação por feedback negativo e pode evitar a degeneração do disco intervertebral inibindo a apoptose de células do núcleo pulposo do disco causada por excesso de ERO. Essa informação proporciona uma nova estratégia de intervenção para a prevenção e o tratamento da degeneração do disco intervertebral.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Apoptosis / Oxidative Stress / NF-E2-Related Factor 2 / Intervertebral Disc Degeneration Type of study: Prognostic study Limits: Humans Language: English Journal: Rev Assoc Med Bras (1992) Year: 2020 Type: Article Institution/Affiliation country: Guangdong Medical University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Apoptosis / Oxidative Stress / NF-E2-Related Factor 2 / Intervertebral Disc Degeneration Type of study: Prognostic study Limits: Humans Language: English Journal: Rev Assoc Med Bras (1992) Year: 2020 Type: Article Institution/Affiliation country: Guangdong Medical University/CN